POLR2A Recombinant antibody, PBS Only

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  WB analysis of COS-7 using 84357-1-RR (same clone as 84357-1-PBS)

  COS-7 cells were subjected to SDS PAGE followed by western blot with 84357-1-RR (POLR2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84357-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 84357-1-RR (same clone as 84357-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84357-1-RR (POLR2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84357-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84357-1-RR (same clone as 84357-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POLR2A antibody (84357-1-RR, Clone: 240869E9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84357-1-PBS in a different storage buffer formulation.

POLR2A {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84357-1-PBS

Clone No.240869E9

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:2.7.7.48, DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit A, 3'-5' exoribonuclease

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Product Information

84357-1-PBS targets POLR2A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, monkey samples.

• Tested Reactivity: human, monkey
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• Calculated Molecular Weight: 217 kDa
• Observed Molecular Weight: 245 kDa
• GenBank Accession Number: NM_000937
• Gene Symbol: POLR2A
• Gene ID (NCBI): 5430
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P24928
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

DNA-directed RNA polymerase II subunit RPB1(POLR2A), is part of the core element of the basic RNA polymerase II transcription mechanism. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for the assembly of factors that regulate transcription initiation, elongation, termination, and mRNA processing.