POLR2F Polyclonal antibody

POLR2F Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15334-1-AP

Synonyms

HRBP14.4, POLR2F, POLRF, RPABC14.4, RPABC2, RPB14.4, RPB6, RPB6 homolog, RPC15



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15334-1-AP targets POLR2F in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR2F fusion protein Ag7449
Full Name polymerase (RNA) II (DNA directed) polypeptide F
Calculated molecular weight 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC003582
Gene symbol POLR2F
Gene ID (NCBI) 5435
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for POLR2F antibody 15334-1-APDownload protocol
IHC protocol for POLR2F antibody 15334-1-APDownload protocol
IF protocol for POLR2F antibody 15334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols