POLR2F Polyclonal antibody

• Featured Product
• KD/KO Validated

POLR2F Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15334-1-AP

Synonyms

HRBP14.4, POLR2F, POLRF, RPABC14.4, RPABC2, RPB14.4, RPB6, RPB6 homolog, RPC15

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Validation Data Gallery

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  WB analysis of HeLa using 15334-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15334-1-AP (POLR2F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15334-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15334-1-AP (POLR2F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 15334-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15334-1-AP (POLR2F Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 15334-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15334-1-AP (POLR2F Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 15334-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15334-1-AP (POLR2F Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human cervical cancer using 15334-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 15334-1-AP (POLR2F antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 15334-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 15334-1-AP (POLR2F antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 15334-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15334-1-AP (POLR2F Antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of MCF-7 using 15334-1-AP

  Immunofluorescent analysis of MCF-7 cells, using POLR2F antibody 15334-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human cervical cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

15334-1-AP targets POLR2F in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLR2F fusion protein Ag7449
• Full Name: polymerase (RNA) II (DNA directed) polypeptide F
• Calculated molecular weight: 14 kDa
• Observed molecular weight: 14 kDa
• GenBank accession number: BC003582
• Gene symbol: POLR2F
• Gene ID (NCBI): 5435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity; Authors - Yuanjun Li
mouse	WB	Cell Biosci; Replication and transcription machinery for ranaviruses: components, correlation, and functional architecture.; Authors - Fei Ke ; KD Validated
mouse	WB	Nat Commun; The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors; Authors - Hui Wang
mouse		Genome Biol; Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture; Authors - Yongpeng Jiang