POLR2I Polyclonal antibody

• 

  WB analysis using 17270-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17270-1-AP (POLR2I antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse stomach using 17270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 17270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 17270-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 17270-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17270-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POLR2I antibody (17270-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

POLR2I Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17270-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DNA-directed RNA polymerase II subunit I, DNA-directed RNA polymerase II subunit RPB9, hRPB14.5, RPB14.5, RPB9

---

Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells
• Positive IHC detected in: mouse stomach tissue, rat liver tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

17270-1-AP targets POLR2I in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11027

  Product name: Recombinant human POLR2I protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-125 aa of BC017112

  Sequence: MEPDGTYEPGFVGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE

• Full Name: polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
• Calculated Molecular Weight: 125 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC017112
• Gene Symbol: POLR2I
• Gene ID (NCBI): 5438
• RRID: AB_10836936
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P36954
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POLR2I, also known as RPB9 or hRPB14.5, is a small core subunit of RNA polymerase II involved in DNA repair and many transcription-related processes (PMID: 21450810). It is expressed in many tissues and may play a key role in the pathological process of hypertensive nephropathy (PMID: 34422001).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Mol Cell; Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity; Authors - Yuanjun Li
human	WB	EMBO Rep; The RNA polymerase II subunit Rpb9 activates ATG1 transcription and autophagy; Authors - Ting Huang ; KD Validated