• Featured Product
  • KD/KO Validated

POLR2I Polyclonal antibody

POLR2I Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 17270-1-AP

Synonyms

hRPB14.5, POLR2I, RNA polymerase II subunit B9, RPB14.5, RPB9



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17270-1-AP is POLR2I Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR2I fusion protein Ag11027
Full Name polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
Calculated molecular weight 125 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC017112
Gene symbol POLR2I
Gene ID (NCBI) 5438
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for POLR2I antibody 17270-1-APDownload protocol
IF protocol for POLR2I antibody 17270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity

Authors - Yuanjun Li
humanWB

EMBO Rep

The RNA polymerase II subunit Rpb9 activates ATG1 transcription and autophagy

Authors - Ting Huang
  • KD Validated