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POLR2I Polyclonal antibody
POLR2I Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 17270-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HepG2 cells |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 2 publications below |
Product Information
The immunogen of 17270-1-AP is POLR2I Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | POLR2I fusion protein Ag11027 |
Full Name | polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa |
Calculated molecular weight | 125 aa, 15 kDa |
Observed molecular weight | 15 kDa |
GenBank accession number | BC017112 |
Gene symbol | POLR2I |
Gene ID (NCBI) | 5438 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for POLR2I antibody 17270-1-AP | Download protocol |
IF protocol for POLR2I antibody 17270-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cell Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity | ||
EMBO Rep The RNA polymerase II subunit Rpb9 activates ATG1 transcription and autophagy
|