POLR2I Polyclonal antibody

• Featured Product
• KD/KO Validated

POLR2I Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17270-1-AP

Synonyms

hRPB14.5, POLR2I, RNA polymerase II subunit B9, RPB14.5, RPB9

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Validation Data Gallery

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  WB analysis using 17270-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17270-1-AP (POLR2I antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 17270-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 17270-1-AP (POLR2I antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

The immunogen of 17270-1-AP is POLR2I Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLR2I fusion protein Ag11027
• Full Name: polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
• Calculated molecular weight: 125 aa, 15 kDa
• Observed molecular weight: 15 kDa
• GenBank accession number: BC017112
• Gene symbol: POLR2I
• Gene ID (NCBI): 5438
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity; Authors - Yuanjun Li
human	WB	EMBO Rep; The RNA polymerase II subunit Rpb9 activates ATG1 transcription and autophagy; Authors - Ting Huang ; KD Validated