POLR3A Polyclonal antibody

POLR3A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (2)

Cat no : 17530-1-AP

Synonyms

hRPC155, POLR3A, RNA polymerase III subunit C1, RPC1, RPC155

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Validation Data Gallery

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  WB analysis using 17530-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17530-1-AP (POLR3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 17530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17530-1-AP (POLR3A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 17530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17530-1-AP (POLR3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

17530-1-AP targets POLR3A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLR3A fusion protein Ag11536
• Full Name: polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
• Calculated Molecular Weight: 1390 aa, 156 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC041089
• Gene Symbol: POLR3A
• Gene ID (NCBI): 11128
• RRID: AB_2167983
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF,IP	Cell Rep; Subcellular localization shapes the fate of RNA polymerase III; Authors - Kai Tian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rebecca (Verified Customer) (10-28-2022)

20ug of protein were loaded. Transference was performed at 4ºC and 90V for 2h. Antibody incubation was done ON at 4ºC.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: iPSC-CM

POLR3A Antibody Western Blot validation (1:1000 dilution) in iPSC-CM (Cat no:17530-1-AP)

FH

Krishnendu (Verified Customer) (02-27-2020)

Didn't see any bands even after using different concentrations of lysate.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293T