POLR3F Polyclonal antibody

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  WB analysis of human brain using 16044-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16044-1-AP (POLR3F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 16044-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16044-1-AP (POLR3F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16044-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16044-1-AP (POLR3F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 16044-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16044-1-AP (POLR3F antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

POLR3F Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16044-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

RPC6, RPC39, DNA-directed RNA polymerase III subunit RPC6, DNA-directed RNA polymerase III subunit F

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Tested Applications

• Positive WB detected in: human brain tissue, BxPC-3 cells, HeLa cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16044-1-AP targets POLR3F in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8935

  Product name: Recombinant human POLR3F protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-315 aa of BC012588

  Sequence: MAEVKVKVQPPDADPVEIENRIIELCHQFPHGITDQVIQNEMPHIEAQQRAVAINRLLSMGQLDLLRSNTGLLYRIKDSQNAGKMKGSDNQEKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYMLYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARESKQNPMIQRNSSFASSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYRAVNPIIPPTGLVRAPCGLCPVFDDCHEGGEISPSNCIYMTEWLE

• Full Name: polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa
• Calculated Molecular Weight: 316 aa, 36 kDa
• Observed Molecular Weight: 36-39 kDa
• GenBank Accession Number: BC012588
• Gene Symbol: POLR3F
• Gene ID (NCBI): 10621
• RRID: AB_2165707
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H1D9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.