POM121 Polyclonal antibody

• Featured Product
• KD/KO Validated

POM121 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (2)

Cat no : 15645-1-AP

Synonyms

POM121A, NUP121, Nucleoporin Nup121, Nuclear envelope pore membrane protein POM 121A, Nuclear envelope pore membrane protein POM 121

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Validation Data Gallery

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  WB analysis using 15645-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15645-1-AP (POM121 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 15645-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15645-1-AP (POM121 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 15645-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15645-1-AP (POM121 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 15645-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 15645-1-AP (POM121 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 6 publications below

Product Information

15645-1-AP targets POM121 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POM121 fusion protein Ag8177
• Full Name: POM121 membrane glycoprotein (rat)
• Calculated Molecular Weight: 984 aa, 99 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC008794
• Gene Symbol: POM121
• Gene ID (NCBI): 9883
• RRID: AB_2878160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POM121 is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Catalog#15645-1-AP do well in identifying the nuclear envelope in immunofluorescence experiments.

Publications

Species	Application	Title
human	IF	Nature; Heritable transcriptional defects from aberrations of nuclear architecture; Authors - Stamatis Papathanasiou
human	IF	Nat Neurosci; Interactions between ALS-linked FUS and nucleoporins are associated with defects in the nucleocytoplasmic transport pathway.; Authors - Yen-Chen Lin
human	IF	Dev Cell; A tubule-sheet continuum model for the mechanism of nuclear envelope assembly; Authors - Gengjing Zhao
human	WB	Elife; Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.; Authors - Melissa Kane
human	IHC,WB	Cancer Biomark; POM121 promotes proliferation and metastasis in non-small-cell lung cancer through TGF-β/SMAD and PI3K/AKT pathways; Authors - Lirong Guan ; KD Validated
human	IF	bioRxiv; Altered nuclear envelope homeostasis is a key pathogenic event in C9ORF72-linked ALS/FTD; Authors - Riccardo Sirtori

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

bing (Verified Customer) (08-06-2019)

Works great in Western Blot, I use it for Hela and Vero cells lysate, go dilution 1:5000 in 5% milk.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Hela cells Vero cells

FH

Yen-Chen (Verified Customer) (12-03-2018)

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:400 for IF, 1:2000 for WB
• Cell Tissue Type: human neuron