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  • KD/KO Validated

POM121 Polyclonal antibody

POM121 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 15645-1-AP

Synonyms

POM121A, NUP121, Nucleoporin Nup121, Nuclear envelope pore membrane protein POM 121A, Nuclear envelope pore membrane protein POM 121



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15645-1-AP targets POM121 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POM121 fusion protein Ag8177
Full Name POM121 membrane glycoprotein (rat)
Calculated Molecular Weight 984 aa, 99 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC008794
Gene Symbol POM121
Gene ID (NCBI) 9883
RRIDAB_2878160
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POM121 is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Catalog#15645-1-AP do well in identifying the nuclear envelope in immunofluorescence experiments.

Protocols

Product Specific Protocols
WB protocol for POM121 antibody 15645-1-APDownload protocol
IHC protocol for POM121 antibody 15645-1-APDownload protocol
IF protocol for POM121 antibody 15645-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nature

Heritable transcriptional defects from aberrations of nuclear architecture

Authors - Stamatis Papathanasiou
humanIF

Nat Neurosci

Interactions between ALS-linked FUS and nucleoporins are associated with defects in the nucleocytoplasmic transport pathway.

Authors - Yen-Chen Lin
humanIF

Dev Cell

A tubule-sheet continuum model for the mechanism of nuclear envelope assembly

Authors - Gengjing Zhao
humanWB

Elife

Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.

Authors - Melissa Kane
humanIHC,WB

Cancer Biomark

POM121 promotes proliferation and metastasis in non-small-cell lung cancer through TGF-β/SMAD and PI3K/AKT pathways

Authors - Lirong Guan
  • KD Validated
humanIF

bioRxiv

Altered nuclear envelope homeostasis is a key pathogenic event in C9ORF72-linked ALS/FTD

Authors - Riccardo Sirtori

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

bing (Verified Customer) (08-06-2019)

Works great in Western Blot, I use it for Hela and Vero cells lysate, go dilution 1:5000 in 5% milk.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Hela cells Vero cells
FH

Yen-Chen (Verified Customer) (12-03-2018)

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:400 for IF, 1:2000 for WB
  • Cell Tissue Type: human neuron