POMC Monoclonal antibody

POMC Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3C12C7

Cat no : 66358-1-Ig

Synonyms

ACTH, ACTH Melanotropin alpha, Adrenocorticotropic hormone, Beta LPH Lipotropin gamma, CLIP, CLIP Lipotropin beta, Corticotropin lipotropin, Gamma LPH Melanotropin beta, LPH, MSH, POC, POMC, Pro opiomelanocortin, proopiomelanocortin



Tested Applications

Positive WB detected inpig brain tissue, rat brain tissue, A375 cells, human placenta tissue, PC-12 cells
Positive IHC detected inhuman pituitary tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

66358-1-Ig targets POMC in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse, pig samples.

Tested Reactivity human, rat, mouse, pig
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen POMC fusion protein Ag17311
Full Name proopiomelanocortin
Calculated molecular weight 267 aa, 29 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC065832
Gene symbol POMC
Gene ID (NCBI) 5443
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.

Protocols

Product Specific Protocols
WB protocol for POMC antibody 66358-1-IgDownload protocol
IHC protocol for POMC antibody 66358-1-IgDownload protocol
IF protocol for POMC antibody 66358-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Biomed Pharmacother

919 syrup inhibits ROS-mediated leptin-induced anorexia by activating PPARγ and improves gut flora abnormalities.

Authors - Jing-Wei Xing