PON2 Polyclonal antibody, PBS Only

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• PON2 Polyclonal antibody, PBS Only (14379-1-PBS)

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  WB analysis of human liver using 14379-1-AP (same clone as 14379-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 14379-1-AP (PON2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14379-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 14379-1-AP (same clone as 14379-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 14379-1-AP (PON2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14379-1-PBS in a different storage buffer formulation.

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  IP experiment of L02 using 14379-1-AP (same clone as 14379-1-PBS)

  IP result of anti-PON2 (IP:14379-1-AP, 4ug; Detection:14379-1-AP 1:500) with L02 cells lysate 3200ug. This data was developed using the same antibody clone with 14379-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14379-1-AP (same clone as 14379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14379-1-AP (PON2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14379-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14379-1-AP (same clone as 14379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14379-1-AP (PON2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14379-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14379-1-AP (same clone as 14379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14379-1-AP (PON2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14379-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14379-1-AP (same clone as 14379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14379-1-AP (PON2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14379-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14379-1-AP (same clone as 14379-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using PON2 antibody 14379-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14379-1-PBS in a different storage buffer formulation.

PON2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 14379-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

14379-1-PBS targets PON2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5759

  Product name: Recombinant human PON2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-219 aa of BC046160

  Sequence: MGRLVAVGLLGIALALLGERLLALRNRLKASREVESVDLPHCHLIKGIEAGSEDIDILPNGLAFFSVGLKFPGLHSFAPDKPGGILMMDLKEEKPRARELRISRGFDLASFNPHGISTFIDNDDTVYLFVVNHPEFKNTVEIFKFEEGENSLLHLKTVKHELLPSVNDITAVGPAHFYATNDHYFSDPFLKYLETYLNLHWANVVYYSPNEVKVVYLCC

• Full Name: paraoxonase 2
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC046160
• Gene Symbol: PON2
• Gene ID (NCBI): 5445
• RRID: AB_2166330
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15165
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PON2(Serum paraoxonase/arylesterase 2) has antioxidant activity and can prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Highest levels of PON2 protein are found in the mouse lung and small intestine, followed by the heart and liver, while lower levels are present in the testis, kidney and brain. PON2 expression in tissues from female mice is always significantly higher than in male animals. There are also some reports showing two bands of 43 kDa and 53 kDa to be detected through western blot as the two isoforms of this protein. (PMID:21354197).