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  • Western Blot (WB) analysis of HepG2 cells using POP4 Polyclonal antibody (15273-1-AP)

    WB analysis of HepG2 using 15273-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15273-1-AP (POP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using POP4 Polyclonal antibody (15273-1-AP)

    WB analysis of HeLa using 15273-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15273-1-AP (POP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POP4 Polyclonal antibody (15273-1-AP)

    IF Staining of HepG2 using 15273-1-AP

    Immunofluorescent analysis of HepG2 cells using 15273-1-AP (POP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

POP4 Polyclonal antibody

POP4 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15273-1-AP
Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

RPP29, POP 4, hPOP4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15273-1-AP targets POP4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POP4 fusion protein Ag7387 Predict reactive species
Full Name processing of precursor 4, ribonuclease P/MRP subunit (S. cerevisiae)
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC004438
Gene Symbol POP4
Gene ID (NCBI) 10775
RRIDAB_2284033
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95707
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POP4 antibody 15273-1-APDownload protocol
IF protocol for POP4 antibody 15273-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Arnaud (Verified Customer) (07-03-2025)

Works well

  • Applications: Western Blot
  • Cell Tissue Type: JLat