POP4 Polyclonal antibody

POP4 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 15273-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

RPP29, POP 4, hPOP4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIF/ICC

WB analysis of HepG2 using 15273-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15273-1-AP (POP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 15273-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15273-1-AP (POP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 15273-1-AP
Immunofluorescent analysis of HepG2 cells using 15273-1-AP (POP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	HepG2 cells, HeLa cells
Positive IF/ICC detected in	HepG2 cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

15273-1-AP targets POP4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7387 Product name: Recombinant human POP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-220 aa of BC004438 Sequence: MKSVIYHALSQKEANDSDVQPSGAQRAEAFVRAFLKRSTPRMSPQAREDQLQRKAVVLEYFTRHKRKEKKKKAKGLSARQRRELRLFDIKPEQQRYSLFLPLHELWKQYIRDLCSGLKPDTQPQMIQAKLLKADLHGAIISVTKSKCPSYVGITGILLQETKHIFKIITKEDRLKVIPKLNCVFTVETDGFISYIYGSKFQLRSSERSAKKFKAKGTIDL Predict reactive species
Full Name	processing of precursor 4, ribonuclease P/MRP subunit (S. cerevisiae)
Calculated Molecular Weight	25 kDa
Observed Molecular Weight	29 kDa
GenBank Accession Number	BC004438
Gene Symbol	POP4
Gene ID (NCBI)	10775
RRID	AB_2284033
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95707
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.