POP4 Polyclonal antibody

POP4 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 15273-1-AP

Synonyms

hPOP4, POP4, RPP29

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Validation Data Gallery

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  WB analysis of HepG2 using 15273-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15273-1-AP (POP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15273-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15273-1-AP (POP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 15273-1-AP

  Immunofluorescent analysis of HepG2 cells using 15273-1-AP (POP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15273-1-AP targets POP4 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POP4 fusion protein Ag7387
• Full Name: processing of precursor 4, ribonuclease P/MRP subunit (S. cerevisiae)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC004438
• Gene Symbol: POP4
• Gene ID (NCBI): 10775
• RRID: AB_2284033
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.