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POPDC3 Polyclonal antibody

POPDC3 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 11800-1-AP

Synonyms

POP3, POPDC3, popeye domain containing 3, Popeye protein 3, RP11 99L11.2



Tested Applications

Positive WB detected inCOLO 320 cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse heart tissue, human testis tissue, human placenta tissue, human skeletal muscle tissue, human lung tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11800-1-AP targets POPDC3 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POPDC3 fusion protein Ag2375
Full Name popeye domain containing 3
Calculated molecular weight 34 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC022323
Gene symbol POPDC3
Gene ID (NCBI) 64208
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Popeye domain-containing (POPDC) gene family includes BVES/POPDC1, POPDC2 and POPDC3. POPDC proteins are highly evolutionarily conserved transmembrane proteins expressed in embryonic epithelium, heart and muscle. POPDC3 is a 291-amino acid protein containing three putative transmembrane domains. It has been reported that POPDC3 expression is reduced in gastric cancer, suggesting it might play an important role in the progression and metastases of gastric cancer. (PMID: 10882522; 20627872; 22654436)

Protocols

Product Specific Protocols
WB protocol for POPDC3 antibody 11800-1-APDownload protocol
IHC protocol for POPDC3 antibody 11800-1-APDownload protocol
IP protocol for POPDC3 antibody 11800-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

World J Gastroenterol

Reduced Popdc3 expression correlates with high risk and poor survival in patients with gastric cancer.

Authors - Luo Deng D
humanWB,IHC

Carcinogenesis

Frequent silencing of popeye domain-containing genes, BVES and POPDC3, is associated with promoter hypermethylation in gastric cancer.

Authors - Kim Mirang M
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