|Positive WB detected in||COLO 320 cells, HEK-293 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||mouse heart tissue, human testis tissue, human placenta tissue, human skeletal muscle tissue, human lung tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11800-1-AP targets POPDC3 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||POPDC3 fusion protein Ag2375|
|Full Name||popeye domain containing 3|
|Calculated molecular weight||34 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||BC022323|
|Gene ID (NCBI)||64208|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The Popeye domain-containing (POPDC) gene family includes BVES/POPDC1, POPDC2 and POPDC3. POPDC proteins are highly evolutionarily conserved transmembrane proteins expressed in embryonic epithelium, heart and muscle. POPDC3 is a 291-amino acid protein containing three putative transmembrane domains. It has been reported that POPDC3 expression is reduced in gastric cancer, suggesting it might play an important role in the progression and metastases of gastric cancer. (PMID: 10882522; 20627872; 22654436)
World J Gastroenterol
Reduced Popdc3 expression correlates with high risk and poor survival in patients with gastric cancer.
Frequent silencing of popeye domain-containing genes, BVES and POPDC3, is associated with promoter hypermethylation in gastric cancer.