Oct6-Specific Polyclonal antibody

Oct6-Specific Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 18997-1-AP

Synonyms

Oct 6, Oct6, Octamer binding protein 6, OTF 6, OTF6, POU class 3 homeobox 1, POU3F1, SCIP

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Validation Data Gallery

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  WB analysis of HEK-293 using 18997-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18997-1-AP (Oct6-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18997-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18997-1-AP (Oct6-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

18997-1-AP targets Oct6-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: POU class 3 homeobox 1
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: NM_002699
• Gene Symbol: POU3F1
• Gene ID (NCBI): 5453
• RRID: AB_2167357
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OCT6, also named as POU3F1 and OTF6, belongs to the POU transcription factor family and Class-3 subfamily. OCT6 is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). It is thought to be involved in early embryogenesis and neurogenesis. The antibody is specific to OCT6.

Publications

Species	Application	Title
mouse	WB	Dev Cell; DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice; Authors - Dingfeng Zou
rat	WB,IF	Neurochem Res; Runx2 was Correlated with Neurite Outgrowth and Schwann Cell Differentiation, Migration After Sciatic Nerve Crush.; Authors - Dazhi Ding
mouse	WB,IHC	Cell Rep; Nucleoporin Seh1 maintains Schwann cell homeostasis by regulating genome stability and necroptosis; Authors - Mei Wu