BRN2-Specific Polyclonal antibody

BRN2-Specific Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA, IF

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 18998-1-AP

Synonyms

BRN2, POU3F2, Brn-2, Brn 2, Brain-specific homeobox/POU domain protein 2

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Validation Data Gallery

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  WB analysis of HeLa using 18998-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18998-1-AP (BRN2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 18998-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 18998-1-AP (BRN2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18998-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18998-1-AP (BRN2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 18998-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18998-1-AP (BRN2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18998-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18998-1-AP (BRN2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HEK-293 using 18998-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human BRN2-Specific (18998-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HeLa cells, A375 cells, HEK-293 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

18998-1-AP targets BRN2-Specific in WB, IHC, FC (Intra), ELISA, IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: POU class 3 homeobox 2
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: NM_005604
• Gene Symbol: BRN2
• Gene ID (NCBI): 5454
• RRID: AB_10597389
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. It shares a highly homologous region that referred to as the POU domain. POU3F2 is a Class III POU genes that expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression. POU3F2 is required for maintaining neural cell differetiation and has a role in the production and positioning of neocortical neurons.

Publications

Species	Application	Title
human	WB	Genes Dev; Protein neddylation as a therapeutic target in pulmonary and extrapulmonary small cell carcinomas.; Authors - Justin P Norton
rat	WB	CNS Neurosci Ther; Reactive astrocytes targeting with oral vitamin A: Efficient neuronal regeneration for Parkinson's disease treatment and reversal of associated liver fibrosis; Authors - Nesrine Saeid El-Mezayen
	IF	Elife; Human-specific ARHGAP11B induces hallmarks of neocortical expansion in developing ferret neocortex.; Authors - Nereo Kalebic

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Etienne (Verified Customer) (12-19-2022)

Good antibody, worked best at 1/200

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/50, 1/200, 1/500
• Cell Tissue Type: Human Brain Organoids

BRN2-Specific Antibody Immunofluorescence validation (1/50, 1/200, 1/500 dilution) in Human Brain Organoids (Cat no:18998-1-AP)