POU3F3-Specific Polyclonal antibody

POU3F3-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 18999-1-AP

Synonyms

Brain 1, Brn 1, BRN1, Oct 8, Octamer binding protein 8, OTF 8, OTF8, POU class 3 homeobox 3, POU3F3

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 18999-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 18999-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 18999-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18999-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18999-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18999-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18999-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18999-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18999-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 18999-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 18999-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18999-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 18999-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 18999-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HEK-293 cells, human brain tissue, mouse brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

18999-1-AP targets POU3F3-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: POU class 3 homeobox 3
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: NM_006236
• Gene Symbol: POU3F3
• Gene ID (NCBI): 5455
• RRID: AB_2284130
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OTF8, also named as POU3F3 and BRN1, belongs to the POU transcription factor family and Class-3 subfamily. The antibody is specific to OTF8.

Publications

Species	Application	Title
mouse	WB	Dev Cell; AP-2β/KCTD1 Control Distal Nephron Differentiation and Protect against Renal Fibrosis.; Authors - Alexander G Marneros
human	WB	Int Immunopharmacol; Iguratimod-encapsulating PLGA-NPs induce human multiple myeloma cell death via reactive oxygen species and Caspase-dependent signalling.; Authors - Faming Wang
human	IHC	Histochem Cell Biol; Connecting tubules develop from the tip of the ureteric bud in the human kidney.; Authors - Beatrix Sarkany