Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, fetal human brain tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18999-1-AP targets POU3F3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | POU class 3 homeobox 3 |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 66-70 kDa |
| GenBank Accession Number | NM_006236 |
| Gene Symbol | POU3F3 |
| Gene ID (NCBI) | 5455 |
| RRID | AB_2284130 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20264 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
POU3F3, also referred to as OTF8, Brain-1, encodes a member of class III of the POU family of transcription factors, is a well-known transcription factor involved in the development of the central nervous system. POU3F3 is composed by two functional domains needed for the site-specific binding to its target genes: the POU-specific (POU-S) domain and the homeobox domain (POU-H) (PMID: 37165752). Endogenous POU3F3 is upregulated in NSCLC tissues and cells, and high expression of POU3F3 correlated with poor survival prognosis (PMID: 39932442). The antibody is specific to OTF8.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for POU3F3 antibody 18999-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
POU3F3 Polyclonal antibody (Cat# 18999-1-AP) has 6 total citations published in Advanced Science, Cell Communication and Signaling, Developmental Cell, International Immunopharmacology, Histochemistry and Cell Biology, and Journal of Human Genetics. The associated research fields span non-small cell lung cancer proliferation, hepatocellular carcinoma ferroptosis resistance, renal fibrosis and nephron differentiation, multiple myeloma apoptosis, kidney development, and neurodevelopmental disorders.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Phosphorylation of POU3F3 Mediated Nuclear Translocation Promotes Proliferation in Non-Small Cell Lung Cancer through Accelerating ATP5PF Transcription and ATP Production | ||
Cell Commun Signal In vivo CRISPR screening identifies POU3F3 as a novel regulator of ferroptosis resistance in hepatocellular carcinoma via retinoic acid signaling | ||
Dev Cell AP-2β/KCTD1 Control Distal Nephron Differentiation and Protect against Renal Fibrosis. | ||
Int Immunopharmacol Iguratimod-encapsulating PLGA-NPs induce human multiple myeloma cell death via reactive oxygen species and Caspase-dependent signalling. | ||
Histochem Cell Biol Connecting tubules develop from the tip of the ureteric bud in the human kidney. | ||
J Hum Genet Identification of 5' untranslated region variants in genes involved in neurodevelopmental disorders |
