POU4F3 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82980-1-RR (same clone as 82980-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82980-1-RR (POU4F3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82980-1-RR (same clone as 82980-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82980-1-RR (POU4F3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82980-1-RR (same clone as 82980-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82980-1-RR (POU4F3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 82980-1-RR (same clone as 82980-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using POU4F3 antibody (82980-1-RR, Clone: 230345F1 ) at dilution of 1:350 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 82980-1-RR (same clone as 82980-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using POU4F3 antibody (82980-1-RR, Clone: 230345F1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

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  FC experiment of U-251 using 82980-1-RR (same clone as 82980-1-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human POU4F3 (82980-1-RR, Clone:230345F1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 82980-1-PBS

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug POU4F3 Recombinant monoclonal antibody, PBS Only (82980-1-PBS, Clone:230345F1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 82980-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82980-1-RR against Human POU4F3 were performed. The affinity constant is 0.234 nM.

POU4F3 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Cat No. 82980-1-PBS

Clone No.230345F1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Synonyms

230345F1, Brain 3C, Brain-3C, Brain-specific homeobox/POU domain protein 3C, Brn 3C

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Tested Applications

• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82980-1-PBS targets POU4F3 in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag16004

  Product name: Recombinant human POU4F3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 99-184 aa of BC104923

  Sequence: HPAALTSHPHHAVHQGLEGDLLEHISPTLSVSGLGAPEHSVMPAQIHPHHLGAMGHLHQAMGMSHPHTVAPHSAMPACLSDVESDP

• Full Name: POU class 4 homeobox 3
• Calculated Molecular Weight: 338 aa, 37 kDa
• Observed Molecular Weight: 33-40 kDa
• GenBank Accession Number: BC104923
• Gene Symbol: POU4F3
• Gene ID (NCBI): 5459
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15319
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.