PPID Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84115-1-RR (same clone as 84115-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84115-1-RR (PPID antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84115-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 84115-1-RR (same clone as 84115-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug PPID Recombinant Antibody (84115-1-RR, Clone:241361B2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84115-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84115-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 84115-1-PBS against Human PPID was performed. The affinity constant is below 1 pM.

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  Affinity and Kinetic Characterization of 84115-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84115-1-RR against Human PPID were performed. The affinity constant is 0.313 nM.

PPID Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 84115-1-PBS

Clone No.241361B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

241361B2, 40 kDa peptidyl-prolyl cis-trans isomerase, Cyclophilin 40, Cyclophilin D, Cyclophilin-40

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Product Information

84115-1-PBS targets PPID in WB, FC (Intra), Cytometric bead array, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3409

  Product name: Recombinant human PPID protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 57-370 aa of BC030707

  Sequence: GIGHTTGKPLHFKGCPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGRNTNGSQFFITTVPTPHLDGKHVVFGQVIKGIGVARILENVEVKGEKPAKLCVIAECGELKEGDDGGIFPKDGSGDSHPDFPEDADIDLKDVDKILLITEDLKNIGNTFFKSQNWEMAIKKYAEVLRYVDSSKAVIETADRAKLQPIALSCVLNIGACKLKMSNWQGAIDSCLEALELDPSNTKALYRRAQGWQGLKEYDQALADLKKAQGIAPEDKAIQAELLKVKQKIKAQKDKEKAVYAKMFA

• Full Name: peptidylprolyl isomerase D
• Calculated Molecular Weight: 370 aa, 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC030707
• Gene Symbol: PPID
• Gene ID (NCBI): 5481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q08752
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228).