Tested Applications
| Positive WB detected in | BxPC-3 cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15357-1-AP targets Cyclophilin E in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7603 Product name: Recombinant human PPIE protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC004898 Sequence: MATTKRVLYVGGLAEEVDDKVLHAAFIPFGDITDIQIPLDYETEKHRGFAFVEFELAEDAAAAIDNMNESELFGRTIRVNLAKPMRIKEGSSRPVWSDDDWLKKFSGKTLEENKEEEGSEPPKAETQEGEPIAKKARSNPQVYMDIKIGNKPAGRIQMLLRSDVVPMTAENFRCLCTHEKGFGFKGSSFHRIIPQFMCQGGDFTNHNGTGGKSIYGKKFDDENFILKHTGPGLLSMANSGPNTNGSQFFLTCDKTDWLDGKHVVFGEVTEGLDVLRQIEAQGSKDGKPKQKVIIADCGEYV Predict reactive species |
| Full Name | peptidylprolyl isomerase E (cyclophilin E) |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 33-38 kDa |
| GenBank Accession Number | BC004898 |
| Gene Symbol | Cyclophilin E |
| Gene ID (NCBI) | 10450 |
| RRID | AB_2878131 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UNP9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cyclophilin E (CypE), also known as Cyclophilin-33, Rotamase E, CYP33, PPIE, is an enzyme which belongs to the cyclophilin-type PPIase family of PPIase E subfamily (PMID: 15998457). Cyclophilin E negatively regulates influenza virus replication and transcription occurs by impairing the formation of the vRNP (PMID: 21887220). Additionally, Cyclophilin E plays an important role in osteoblast differentiation as a positive regulator by increasing the transcriptional activity of Runx2 (PMID: 37947627).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Cyclophilin E antibody 15357-1-AP | Download protocol |
| IHC protocol for Cyclophilin E antibody 15357-1-AP | Download protocol |
| WB protocol for Cyclophilin E antibody 15357-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Cyclophilin E (PPIE) Polyclonal antibody (Cat# 15357-1-AP) has 1 citation to date. It appears in Molecular Cell (2026), where it was used in Western blot to study a dual-module ECAS complex coupling transcription elongation and RNA processing in human samples. The associated research field is transcriptional regulation and RNA processing.
| Species | Application | Title |
|---|---|---|
Reviews
What customers say
Across three Western Blot reviews (ratings of 4, 3, and 4 out of 5), users tested the antibody in RKO, HEK293T, and mESC cells at dilutions of 1:2000–1:3000. The expected ~33 kDa band (PPIE/Cyclophilin E) was consistently detected, though reviewers noted it was not always the strongest signal. A recurring concern was the presence of multiple non-specific bands, which slightly reduced confidence in specificity. Overall, the antibody performs adequately for target detection but may benefit from optimization to minimize background.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Aleksandr (Verified Customer) (07-19-2025) | Overall antibody recognize the band of the expected size, but there are several non-specific bands
 |
Moritz (Verified Customer) (09-12-2024) | Several bands were observable in WB, also the expected size of PPIE, although it was not the strongest.
 |
Tsimafei (Verified Customer) (08-12-2024) | Multiple bands are present, however the one corresponding to Cyclophilin E size (~33 kDa) is clearly visible
 |
