Cyclophilin E Polyclonal antibody

Cyclophilin E Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Reviews (2)

Cat no : 15357-1-AP

Synonyms

PPIE, Peptidyl-prolyl cis-trans isomerase E, EC:5.2.1.8, CYP33, CYP 33

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Validation Data Gallery

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  WB analysis of HEK-293 using 15357-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15357-1-AP (Cyclophilin E antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15357-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15357-1-AP (Cyclophilin E antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15357-1-AP targets Cyclophilin E in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclophilin E fusion protein Ag7603
• Full Name: peptidylprolyl isomerase E (cyclophilin E)
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC004898
• Gene Symbol: Cyclophilin E
• Gene ID (NCBI): 10450
• RRID: AB_2878131
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Moritz (Verified Customer) (09-12-2024)

Several bands were observable in WB, also the expected size of PPIE, although it was not the strongest.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293T

Cyclophilin E Antibody Western Blot validation (1:3000 dilution) in HEK293T (Cat no:15357-1-AP)

FH

Tsimafei (Verified Customer) (08-12-2024)

Multiple bands are present, however the one corresponding to Cyclophilin E size (~33 kDa) is clearly visible

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

Cyclophilin E Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:15357-1-AP)