Tested Applications
| Positive WB detected in | Jurkat cells, NIH/3T3 cells, C6 cells, PC-12 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12985-1-AP targets PPIG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3622 Product name: Recombinant human PPIG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-355 aa of BC001555 Sequence: MGIKVQRPRCFFDIAINNQPAGRVVFELFSDVCPKTCENFRCLCTGEKGTGKSTQKPLHYKSCLFHRVVKDFMVQGGDFSEGNGRGGESIYGGFFEDESFAVKHNKEFLLSMANRGKDTNGSQFFITTKPTPHLDGHHVVFGQVISGQEVVREIENQKTDAASKPFAEVRILSCGELIPKSKVKKEEKKRHKSSSSSSSSSSDSDSSSDSQSSSDSSDSESATEEKSKKRKKKHRKNSRKHKKEKKKRKKSKKSASSESEAENLEAQPQSTVRPEEIPPIPENRFLMRKSPPKADEKERKNRERERERECNPPNSQPASYQRRLLVTRSGRKIKGRGPRVGDSFPRDLHNIAFVF Predict reactive species |
| Full Name | peptidylprolyl isomerase G (cyclophilin G) |
| Calculated Molecular Weight | 739 aa, 87 kDa |
| Observed Molecular Weight | 89 kDa |
| GenBank Accession Number | BC001555 |
| Gene Symbol | PPIG |
| Gene ID (NCBI) | 9360 |
| RRID | AB_2169281 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13427 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Peptidyl-prolyl cis-trans isomerase G (commonly abbreviated as PPIG) belongs to the cyclophilin subfamily of peptidyl-prolyl cis-trans isomerases (PPIases), which act as molecular chaperones to accelerate protein folding and regulate vital cellular processes.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PPIG antibody 12985-1-AP | Download protocol |
| IHC protocol for PPIG antibody 12985-1-AP | Download protocol |
| WB protocol for PPIG antibody 12985-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PPIG Polyclonal antibody (Cat# 12985-1-AP) has 1 citation. It was used in an immunofluorescence (IF) study published in Science Advances (2024) by Joonwon Kim et al., titled "High-throughput tagging of endogenous loci for rapid characterization of protein function." The research field encompasses high-throughput genomics, endogenous protein tagging, and functional proteomics in human cells.
| Species | Application | Title |
|---|---|---|
Sci Adv High-throughput tagging of endogenous loci for rapid characterization of protein function |
Reviews
What customers say
The single review for this product is a 5-star rating. The user performed immunocytochemistry on HeLa cells using a 1:1000 dilution in 5% goat serum/PBST with a 2-hour room-temperature incubation, reporting good nuclear staining. Overall, the feedback is positive, highlighting reliable performance and clear staining results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tatyana (Verified Customer) (12-22-2024) | ICC with antibody diluted in 5% goat serum in PBST, with 2 h incubation at RT. Good nuclear staining
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