PPIL2 Polyclonal antibody, PBS Only

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• PPIL2 Polyclonal antibody, PBS Only (12590-2-PBS)

PPIL2 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 12590-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Cyclophilin-60, Cyclophilin-like protein Cyp-60, Cyp-60, EC:2.3.2.27, hCyP-60

• 

  WB analysis of human kidney using 12590-2-AP (same clone as 12590-2-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12590-2-AP (PPIL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12590-2-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 12590-2-AP (same clone as 12590-2-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12590-2-AP (PPIL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12590-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 12590-2-AP (same clone as 12590-2-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12590-2-AP (PPIL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12590-2-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 12590-2-AP (same clone as 12590-2-PBS)

  IP result of anti-PPIL2 (IP:12590-2-AP, 4ug; Detection:12590-2-AP 1:300) with HEK-293 cells lysate 2600 ug. This data was developed using the same antibody clone with 12590-2-PBS in a different storage buffer formulation.

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Product Information

12590-2-PBS targets PPIL2 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3296

  Product name: Recombinant human PPIL2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 221-520 aa of BC028385

  Sequence: LAATMKAPEKKKVDKLNAAHYSTGKVSASFTSTAMVPETTHEAAAIDEDVLRYQFVKKKGYVRLHTNKGDLNLELHCDLTPKTCENFIRLCKKHYYDGTIFHRSIRNFVIQGGDPTGTGTGGESYWGKPFKDEFRPNLSHTGRGILSMANSGPNSNRSQFFITFRSCAYLDKKHTIFGRVVGGFDVLTAMENVESDPKTDRPKEEIRIDATTVFVDPYEEADAQIAQERKTQLKIAPETKVKSSQPQAGSQGPQTFRQGVGKYINPAATKRAAEEEPSTSATVPMSKKKPSRGFGDFSSW

• Full Name: peptidylprolyl isomerase (cyclophilin)-like 2
• Calculated Molecular Weight: 520 aa, 59 kDa
• Observed Molecular Weight: 60~70 kDa
• GenBank Accession Number: BC028385
• Gene Symbol: PPIL2
• Gene ID (NCBI): 23759
• RRID: AB_2169606
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13356
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Peptidylprolyl isomerase (cyclophilin)-like 2 (PPIL2), also known as Cyclophilin-like protein Cyp-60, is a U-box-type E3 ubiquitin ligase belonging to the cyclophilin family. PPIL2 is also a target of the JAK2/STAT5 pathway and upregulated in patients with MPNs (PMID: 36092721, 25992900). PPIL2 is a large ~60 kDa (520 amino acids) protein that has a central cyclophilin-like domain and also has an N-terminal U-box domain that exhibits E3 ligase activity. Western detected PPIL2 at an apparent molecular mass of 60~70 kDa (PMID: 40338661, 29352246).