PPIL6 Polyclonal antibody

PPIL6 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17452-1-AP

Synonyms

Rotamase PPIL6, Probable inactive peptidyl-prolyl cis-trans isomerase-like 6, PPIase, Cyclophilin-like protein PPIL6, Cyclophilin like protein PPIL6

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Validation Data Gallery

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  WB analysis of HeLa using 17452-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17452-1-AP (PPIL6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17452-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17452-1-AP (PPIL6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 17452-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17452-1-AP (PPIL6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

17452-1-AP targets PPIL6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPIL6 fusion protein Ag11578
• Full Name: peptidylprolyl isomerase (cyclophilin)-like 6
• Calculated Molecular Weight: 311 aa, 35 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC038716
• Gene Symbol: PPIL6
• Gene ID (NCBI): 285755
• RRID: AB_2237569
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Curr Biol; CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement; Authors - Chao Liu
mouse	WB	Cell Mol Life Sci; CCDC146 is required for sperm flagellum biogenesis and male fertility in mice; Authors - Yanjie Ma