PPIL6 Polyclonal antibody

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  WB analysis of HeLa using 17452-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17452-1-AP (PPIL6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17452-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17452-1-AP (PPIL6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 17452-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17452-1-AP (PPIL6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PPIL6 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 17452-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Cyclophilin like protein PPIL6, Cyclophilin-like protein PPIL6, PPIase, Probable inactive peptidyl-prolyl cis-trans isomerase-like 6, Rotamase PPIL6

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

17452-1-AP targets PPIL6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11578

  Product name: Recombinant human PPIL6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-311 aa of BC038716

  Sequence: MARPQPCGPPHARCGSPSLPERPLQVKVVGLFSCPNFQIAKSAAENLKNNHPSKFEDPILVPLQEFAWHQYLQEKKRELKNETWEYSSSVISFVNGQFLGDALDLQKWAHEVWDIVDIKPSALYDALTEDFSAKFLRDTKHDFVFLDICIDSSPIGRLIFELYCDVCPKTCKNFQVLCTGKAGFSQRGIRLHYKNSIFHRIVQNGWIQGGDIVYGKGDNGESIYGPTFEDENFSVPHNKRGVLGMANKGRHSNGSQFYITLQATPYLDRKFVAFGQLIEGTEVLKQLELVPTQNERPIHMCRITDSGDPYA

• Full Name: peptidylprolyl isomerase (cyclophilin)-like 6
• Calculated Molecular Weight: 311 aa, 35 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC038716
• Gene Symbol: PPIL6
• Gene ID (NCBI): 285755
• RRID: AB_2237569
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IXY8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peptidylprolyl isomerase-like 6 (PPIL6) is a member of the cyclophilin (peptidyl-prolyl cis-trans isomerase, PPIase) family, proteins that typically catalyze proline residue isomerization and contribute to protein folding and conformational regulation. However, PPIL6 is considered a divergent or atypical cyclophilin, as current evidence suggests it may have limited or altered enzymatic activity compared with canonical PPIases. In humans, PPIL6 expression appears to be enriched in testis, particularly associated with spermatogenic cells, indicating a potential role in male germ cell development and spermatogenesis. Although its precise molecular function remains poorly defined, PPIL6 has been proposed to participate in protein complex assembly or regulatory processes during germ cell differentiation. To date, there is no well-established signaling pathway or interaction network for PPIL6. Research interest in PPIL6 is mainly within reproductive biology, functional genomics, and protein evolution, with limited direct evidence linking it to specific diseases.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Curr Biol; CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement; Authors - Chao Liu
mouse	WB	Cell Mol Life Sci; CCDC146 is required for sperm flagellum biogenesis and male fertility in mice; Authors - Yanjie Ma