PPM1M Polyclonal antibody

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  WB analysis of human lung using 12104-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12104-1-AP (PPM1M antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 12104-1-AP

  IP result of anti-PPM1M (IP:12104-1-AP, 4ug; Detection:12104-1-AP 1:300) with mouse lung tissue lysate 4000ug.

PPM1M Polyclonal Antibody for WB, IP, ELISA

Cat No. 12104-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

PP2C eta, PP2CE, PP2Ceta, PPM1E, PPM1M, Protein phosphatase 1M

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Tested Applications

• Positive WB detected in: human lung tissue
• Positive IP detected in: mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12104-1-AP targets PPM1M in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2748

  Product name: Recombinant human PPM1M protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-247 aa of BC009644

  Sequence: MGGCTALVAVSLQGKLYMANAGDSRAILVRRDEIRPLSFEFTPETERQRIQQLAFVYPELLAGEFTRLEFPRRLKGDDLGQKVLFRDHHMSGWSYKRVEKSDLKYPLIHGQGRQARLLGTLAVSRGLGDHQLRVLDTNIQLKPFLLSVPQVTVLDVDQLELQEDDVVVMATDGLWDVLSNEQVAWLVRSFLPGNQEDPHRFSKLAQMLIHSTQGKEDSLTEEGQVSYDDVSVFVIPLHSQGQESSDH

• Full Name: protein phosphatase 1M (PP2C domain containing)
• Calculated Molecular Weight: 270 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC009644
• Gene Symbol: PPM1M
• Gene ID (NCBI): 132160
• RRID: AB_2170362
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96MI6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.