PDP1 Polyclonal antibody

PDP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 21176-1-AP

Synonyms

PPM2C, Protein phosphatase 2C, PDPC 1, PDP 1, EC:3.1.3.43

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Validation Data Gallery

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  WB analysis using 21176-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21176-1-AP (PPM2C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21176-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21176-1-AP (PPM2C Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 21176-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21176-1-AP (PPM2C Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 21176-1-AP

  IP result of anti-PDP1 (IP:21176-1-AP, 4ug; Detection:21176-1-AP 1:300) with mouse brain tissue lysate 5200ug.

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  IHC staining of human prostate cancer using 21176-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21176-1-AP (PPM2C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 21176-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21176-1-AP (PPM2C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 21176-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21176-1-AP (PPM2C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 21176-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21176-1-AP (PPM2C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: PC-3 cells, mouse skeletal muscle tissue, mouse brain tissue, mouse heart tissue, mouse liver tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human prostate cancer tissue, human thyroid cancer tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below

Product Information

21176-1-AP targets PDP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDP1 fusion protein Ag15453
• Full Name: protein phosphatase 2C, magnesium-dependent, catalytic subunit
• Calculated Molecular Weight: 537 aa, 61 kDa
• Observed Molecular Weight: 55-61 kDa
• GenBank Accession Number: BC047619
• Gene Symbol: PDP1
• Gene ID (NCBI): 54704
• RRID: AB_2878824
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pyruvate dehydrogenase phosphatase 1 (PDP1) is a key hormone-regulated metabolic enzyme that dephosphorylates and activates pyruvate dehydrogenase (PDH), thereby stimulating the conversion of pyruvate into acetyl-CoA (PMID: 36453802). PDP1 expression is regulated by FLT3-ITD in a context-dependent manner, and that it is centrally involved in the adaptation of AML glucose metabolism to the requirements of proliferation, survival and the therapeutic response to FLT3 inhibition (PMID: 37935978).

Publications

Species	Application	Title
human	IHC	Aging (Albany NY); Identification of immune-related cells and genes in the breast invasive carcinoma microenvironment.; Authors - Xingfeng Chen
human	WB	Curr Med Sci; Salidroside Inhibits Lipopolysaccharide-ethanol-induced Activation of Proinflammatory Macrophages via Notch Signaling Pathway.; Authors - Jian-Sha Li
mouse	WB	J Diabetes Res; Pyruvate-Enriched Oral Rehydration Solution Improves Glucometabolic Disorders in the Kidneys of Diabetic db/db Mice.; Authors - Xiao Meng Zhang
human	WB	Cell Death Dis; Mitochondrial dysfunction induces radioresistance in colorectal cancer by activating [Ca2+]m-PDP1-PDH-histone acetylation retrograde signaling.; Authors - Yingying Shi
mouse	WB	ACS Chem Neurosci; FDCA Attenuates Neuroinflammation and Brain Injury after Cerebral Ischemic Stroke; Authors - Xin Guan
mouse	WB	J Physiol; Monocarboxylate transporter 4 deficiency enhances high-intensity interval training-induced metabolic adaptations in skeletal muscle; Authors - Yuki Tamura