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PPME1 Polyclonal antibody, PBS Only (14435-1-PBS)

Filter: AllWBIHC

WB analysis using 14435-1-AP (same clone as 14435-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 14435-1-AP (same clone as 14435-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 14435-1-AP (same clone as 14435-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.
WB analysis of fetal human brain using 14435-1-AP (same clone as 14435-1-PBS)
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 14435-1-AP (same clone as 14435-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 14435-1-AP (same clone as 14435-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 14435-1-AP (same clone as 14435-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14435-1-AP (PPME1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 14435-1-AP (same clone as 14435-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14435-1-AP (PPME1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 14435-1-AP (same clone as 14435-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14435-1-AP (PPME1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.

PPME1 Polyclonal antibody, PBS Only

Name: PPME1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 14435-1-PBS

PPME1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 14435-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

EC:3.1.1.89, PME 1, PME1, PME-1, PP2593

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

14435-1-PBS targets PPME1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5817 Product name: Recombinant human PPME1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-386 aa of BC050705 Sequence: MSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFSPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRSHGETKVKNPEDLSAETMAKDVGNVVEAMYGDLPPPIMLIGHSMGGAIAVHTASSNLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLENAIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEEGSESISKRKKEDDMETKKDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGVDRLDKDLTIGQMQGKFQMQVLPQCGHAVHEDAPDKVAEAVATFLIRHRFAEPIGGFQCVFPGC Predict reactive species
Full Name	protein phosphatase methylesterase 1
Calculated Molecular Weight	42 kDa
Observed Molecular Weight	44 kDa
GenBank Accession Number	BC050705
Gene Symbol	PPME1
Gene ID (NCBI)	51400
RRID	AB_2284364
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y570
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PPME1 (Protein phosphatase methylesterase 1) is a protein phosphatase 2A (PP2A)-specific methyl esterase that negatively regulates PP2A through demethylation at its carboxy terminal leucine 309 residue (PMID: 24253382). PPME1 plays an important role in the development of cancers and may be a biomarker for pancreatic carcinoma, gastric and lung cancer (PMID: 32351291).