PPP1CB-Specific Polyclonal antibody

PPP1CB-Specific Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10140-2-AP

Synonyms

PP1 Beta, PP 1B, PPP1CB, PPP1CD



Tested Applications

Positive WB detected inhuman brain tissue, NIH/3T3
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10140-2-AP targets PPP1CB-Specific in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP1CB-Specific fusion protein Ag0183
Full Name protein phosphatase 1, catalytic subunit, beta isoform
Calculated molecular weight 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC002697
Gene symbol PPP1CB
Gene ID (NCBI) 5500
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein phosphatase 1 (PP1) is the main eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular processes including protein-protein interactions, gene transcription, cell-cycle progression and apoptosis. It is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. The catalytic subunit of PP1 (PP1c) can interact with amount of regulatory subunits in vivo that target it to particular subcellular locations and regulate its activity towards specific substrates. PPP1CB migrated as a 37kd protein on SDS page. This antibody is specific to PPP1CB.

Protocols

Product Specific Protocols
WB protocol for PPP1CB-Specific antibody 10140-2-APDownload protocol
IHC protocol for PPP1CB-Specific antibody 10140-2-APDownload protocol
IF protocol for PPP1CB-Specific antibody 10140-2-APDownload protocol
IP protocol for PPP1CB-Specific antibody 10140-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB, IP

Clin Exp Pharmacol Physiol

Rescue of cardiac failing and remodeling by inhibition of PP1γ is associated with suppression of the ASF-mediated splicing of CaMKIIδ

Authors - Ru-Jia Liao
humanWB,IHC

Melanoma Res

Differential expression patterns of capping protein, protein phosphatase 1, and casein kinase 1 may serve as diagnostic markers for malignant melanoma.

Authors - Sun Daxin D
humanWB

Am J Physiol Cell Physiol

PP1γ functionally augments the alternative splicing of CaMKIIδ through interacting with ASF.

Authors - Chao Huang