Tested Applications
| Positive WB detected in | A549 cells, BxPC-3 cells, NIH/3T3 cells, SH-SY5Y cells, mouse brain tissue |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55136-1-AP targets PPP1CB-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | protein phosphatase 1, catalytic subunit, beta isoform |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 33-40 kDa |
| GenBank Accession Number | NM_002709 |
| Gene Symbol | PPP1CB |
| Gene ID (NCBI) | 5500 |
| RRID | AB_10837232 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62140 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PPP1CB, also named as PP-1B, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. PPP1CB is involved in regulation of ionic conductances and long-term synaptic plasticity. This antibody is specific to PPP1CB.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PPP1CB-Specific antibody 55136-1-AP | Download protocol |
| IHC protocol for PPP1CB-Specific antibody 55136-1-AP | Download protocol |
| WB protocol for PPP1CB-Specific antibody 55136-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PPP1CB-Specific Polyclonal antibody (Cat# 55136-1-AP) has 4 total citations published in J Adv Res, Cell Reports, PLoS Pathogens, and Experimental Eye Research. The associated research fields span neuroinflammation (LPS-induced signaling), glioblastoma proliferation, hepatitis B virus replication, and diabetic retinopathy pathogenesis.
| Species | Application | Title |
|---|---|---|
J Adv Res Transducin-like enhancer of split 3 protects against lipopolysaccharide-induced inflammation through DDX5-ATF1-PPP2R5A signaling | ||
PLoS Pathog Protein phosphatase 1 catalyzes HBV core protein dephosphorylation and is co-packaged with viral pregenomic RNA into nucleocapsids.
| ||
Exp Eye Res PPP1CA/YAP/GS/Gln/mTORC1 pathway activates retinal Müller cells during diabetic retinopathy. |
Reviews
What customers say
Both reviewers gave 5-star ratings. One user found the antibody works beautifully for Western Blot and Immunofluorescence on mouse oocytes and budding yeast, though they noted it cross-reacts with both PPP1CA and PPP1CB (not PPP1CC), suggesting it is not fully specific to PPP1CB alone. The other user reported excellent performance in Western Blot at a 1:3000 dilution on colon tissue and Caco2 cells. Overall, users are satisfied with the antibody's performance across applications, with one caveat regarding isoform specificity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nikki (Verified Customer) (01-28-2026) | Antibody works beautifully for IIF and WB. However, it is not specific for PPP1CB as advertised. We have found it detects human PPP1CA and PPP1CB (does not detect PPP1CC) via immunoblotting of budding yeast expressing one human PPP1C isoform at a time.
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Balawant (Verified Customer) (08-23-2022) | I have used this antibody for western blot in mice tissue sample (colon tissue). it is working great.
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