PPP1CC Polyclonal antibody

PPP1CC Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11082-1-AP

Synonyms

PP1 Gamma, PP 1G, PPP1CC, PPP1G



Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, MCF-7 cells, L02 cells, HEK-293T cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11082-1-AP targets PPP1CC in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP1CC fusion protein Ag1550
Full Name protein phosphatase 1, catalytic subunit, gamma isoform
Calculated molecular weight 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC014073
Gene symbol PPP1CC
Gene ID (NCBI) 5501
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP1CC, also named as PP-1G, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. It is involved in regulation of ionic conductances and long-term synaptic plasticity. PPP1CC may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.

Protocols

Product Specific Protocols
WB protocol for PPP1CC antibody 11082-1-APDownload protocol
IHC protocol for PPP1CC antibody 11082-1-APDownload protocol
IF protocol for PPP1CC antibody 11082-1-APDownload protocol
IP protocol for PPP1CC antibody 11082-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB, IP

Clin Exp Pharmacol Physiol

Rescue of cardiac failing and remodeling by inhibition of PP1γ is associated with suppression of the ASF-mediated splicing of CaMKIIδ

Authors - Ru-Jia Liao
humanWB

Am J Physiol Cell Physiol

PP1γ functionally augments the alternative splicing of CaMKIIδ through interacting with ASF.

Authors - Chao Huang
humanWB

J Biol Chem

Dephosphorylation activates the interferon-stimulated schlafen family member 11 in the DNA Damage Response.

Authors - Dane Malone

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jua (Verified Customer) (08-01-2018)

  • Applications: Flow Cytometry,
  • Primary Antibody Dilution: Souris têtard isralien