PPP1CC Polyclonal antibody

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  WB analysis using 55150-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55150-1-AP (PPP1CC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 55150-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 55150-1-AP (PPP1CC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55150-1-AP

  IP result of anti-PPP1CC (IP:55150-1-AP, 4ug; Detection:55150-1-AP 1:500) with mouse brain tissue lysate 3440ug.

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  IHC staining of human heart using 55150-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55150-1-AP (PPP1CC antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 55150-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55150-1-AP (PPP1CC antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 55150-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55150-1-AP (PPP1CC antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 55150-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55150-1-AP (PPP1CC antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 55150-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PPP1CC antibody (55150-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PPP1CC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 55150-1-AP

Publications(3)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Serine/threonine-protein phosphatase PP1-gamma catalytic subunit, PP-1G, EC:3.1.3.16

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Tested Applications

• Positive WB detected in: mouse brain tissue, L02 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human heart tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

55150-1-AP targets PPP1CC in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: protein phosphatase 1, catalytic subunit, gamma isoform
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_002710
• Gene Symbol: PPP1CC
• Gene ID (NCBI): 5501
• RRID: AB_10792814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P36873
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP1CC, also named as PP-1G, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. It is involved in regulation of ionic conductances and long-term synaptic plasticity. PPP1CC may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. The antibody is specific to PPP1CC.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Front Cell Dev Biol; PERK Signaling Controls Myoblast Differentiation by Regulating MicroRNA Networks.; Authors - Ye-Ya Tan
mouse	WB	J Biol Chem; Hydrogen sulfide modulates eukaryotic translation initiation factor 2α (eIF2α) phosphorylation status in the integrated stress response pathway.; Authors - Vinita Yadav
human	WB	Cell Death Dis; Genome-wide identification of microRNA targets reveals positive regulation of the Hippo pathway by miR-122 during liver development.; Authors - Yin Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vinita (Verified Customer) (12-04-2018)

• Applications: Western Blot,
• Cell Tissue Type: Hek293