PPP1CC Polyclonal antibody

PPP1CC Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 55150-1-AP

Synonyms

PP1 Gamma, PP 1G, PP1C gamma, PPP1CC, PPP1G



Tested Applications

Positive WB detected inmouse brain tissue, L02 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

55150-1-AP targets PPP1CC in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name protein phosphatase 1, catalytic subunit, gamma isoform
Calculated molecular weight 38 kDa
Observed molecular weight 35 kDa
GenBank accession numberNM_002710
Gene symbol PPP1CC
Gene ID (NCBI) 5501
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP1CC, also named as PP-1G, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. It is involved in regulation of ionic conductances and long-term synaptic plasticity. PPP1CC may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. The antibody is specific to PPP1CC.

Protocols

Product Specific Protocols
WB protocol for PPP1CC antibody 55150-1-APDownload protocol
IHC protocol for PPP1CC antibody 55150-1-APDownload protocol
IF protocol for PPP1CC antibody 55150-1-APDownload protocol
IP protocol for PPP1CC antibody 55150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Biol Chem

Hydrogen sulfide modulates eukaryotic translation initiation factor 2α (eIF2α) phosphorylation status in the integrated stress response pathway.

Authors - Vinita Yadav
mouseWB

Front Cell Dev Biol

PERK Signaling Controls Myoblast Differentiation by Regulating MicroRNA Networks.

Authors - Ye-Ya Tan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Vinita (Verified Customer) (12-04-2018)

  • Applications: Western Blot,
  • Cell Tissue Type: Hek293