|Positive WB detected in||mouse brain tissue, L02 cells, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human heart tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
55150-1-AP targets PPP1CC in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||protein phosphatase 1, catalytic subunit, gamma isoform|
|Calculated molecular weight||38 kDa|
|Observed molecular weight||35 kDa|
|GenBank accession number||NM_002710|
|Gene ID (NCBI)||5501|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
PPP1CC, also named as PP-1G, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. It is involved in regulation of ionic conductances and long-term synaptic plasticity. PPP1CC may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. The antibody is specific to PPP1CC.
|Product Specific Protocols|
|WB protocol for PPP1CC antibody 55150-1-AP||Download protocol|
|IHC protocol for PPP1CC antibody 55150-1-AP||Download protocol|
|IF protocol for PPP1CC antibody 55150-1-AP||Download protocol|
|IP protocol for PPP1CC antibody 55150-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Vinita (Verified Customer) (12-04-2018)