Tested Applications
| Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13366-1-AP targets PPP1R12B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4185 Product name: Recombinant human PPP1R12B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-386 aa of BC034430 Sequence: MAELEHLGGKRAESARMRRAEQLRRWRGSLTEQEPAERRGAGRQPLTRRGSPRVRFEDGAVFLAACSSGDTDEVRKLLARGADINTVNVDGLTALHQACIDENLDMVKFLVENRANVNQQDNEGWTPLHAAASCGYLNIAEYFINHGASVGIVNSEGEVPSDLAEEPAMKDLLLEQVKKQGVDLEQSRKEEEQQMLQDARQWLNSGKIEDVRQARSGATALHVAAAKGYSEVLRLLIQAGYELNVQDYDGWTPLHAAAHWGVKEACSILAEALCDMDIRNKLGQTPFDVADEGLVEHLELLQKKQNVLRSEKETRNKLIESDLNSKIQSGFFKNKEKMLYEEETPKSQEMEEENKESSSSSSEEEEGEDEASESETEKEAVLFWPF Predict reactive species |
| Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 12B |
| Calculated Molecular Weight | 982 aa, 110 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC034430 |
| Gene Symbol | PPP1R12B |
| Gene ID (NCBI) | 4660 |
| RRID | AB_2168445 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60237 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PPP1R12B antibody 13366-1-AP | Download protocol |
| IHC protocol for PPP1R12B antibody 13366-1-AP | Download protocol |
| WB protocol for PPP1R12B antibody 13366-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-PPP1R12B antibody (Cat# 13366-1-AP) has 5 citations across journals including EBioMedicine, Frontiers in Cell and Developmental Biology, Journal of Biological Chemistry, Skeletal Muscle, and Gene. Research fields span substance use disorders and neurotransmission signaling, hepatocellular carcinoma cell proliferation, cardiac myosin phosphorylation in striated muscle, murine muscle membrane interactors, and abdominal aortic aneurysm pathogenesis. Applications cited include WB, IF, and IHC in rat, human, and mouse species.
| Species | Application | Title |
|---|---|---|
EBioMedicine Epistatic evidence for gender-dependant slow neurotransmission signalling in substance use disorders: PPP1R12B versus PPP1R1B. | ||
Front Cell Dev Biol PPP1R12B inhibits cell proliferation by inducing G0/G1 phase arrest via PAK2/β-catenin axis in hepatocellular carcinoma.
| ||
J Biol Chem The MYPT2-regulated striated muscle-specific myosin light chain phosphatase limits cardiac myosin phosphorylation in vivo | ||
Gene Revealing PPP1R12B and COL1A1 as piRNA pathway genes contributing to abdominal aortic aneurysm through integrated analysis and experimental validation. |
Reviews
What customers say
The single review for this product is a 5-star rating. The reviewer reported that the antibody works well for immunoprecipitation in mammalian whole cell lysates, specifically using U2OS and HeLa cells. Overall, the feedback is positive, confirming reliable performance in IP applications with common mammalian cell lines.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shalini (Verified Customer) (08-13-2025) | Works nice in mammalian cells whole cell lysates
|
