Tested Applications
| Positive WB detected in | A2780 cells, MCF-7 cells, HEK-293 cells, SGC-7901 cells, MDA-MB-231 cells, SKOV-3 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IF/ICC detected in | SH-SY5Y cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14634-1-AP targets PPP1R15B in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6213 Product name: Recombinant human PPP1R15B;CReP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 369-713 aa of BC065280 Sequence: TTEVPLALEEESPSEGCPSSEIPMEKEPGEGRISVVDYSYLEGDLPISARPACSNKLIDYILGGASSDLETSSDPEGEDWDEEAEDDGFDSDSSLSDSDLEQDPEGLHLWNSFCSVDPYNPQNFTATIQTAARIVPEEPSDSEKDLSGKSDLENSSQSGSLPETPEHSSGEEDDWESSADEAESLKLWNSFCNSDDPYNPLNFKAPFQTSGENEKGCRDSETPSESIVAISECHTLLSCKVQLLGSQESECPDSVQRDVLSGGRHTHVKRKKVTFLEEVTEYYISGDEDRKGPWEEFARDGCRFQKRIQETEDAIGYCLTFEHRERMFNRLQGTCFKGLNVLKQC Predict reactive species |
| Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 15B |
| Calculated Molecular Weight | 79 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC065280 |
| Gene Symbol | PPP1R15B |
| Gene ID (NCBI) | 84919 |
| RRID | AB_2300036 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5SWA1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein phosphatase 1 regulatory subunit 15B(PPP1R15B) is a member of PPP1R15 family, it forms a complex with protein phosphatase1(PP1) and NCK1/2, maintaining low levels of EIF2S1 phosphorylation in unstressed cells, thus is also named constitutive repressor of eIF2alpha phosphorylation (CReP). PPP1R15B is assoicated in endoplasmic reticulum (ER) stress response resulting to a role in translation regulation. Knock-down of PPP1R15B strongly protected mammalian cells against oxidative stress, peroxynitrite stress, and more modestly against accumulation of malfolded proteins in the ER. Recently, PPP1R15B was found to associate with cell membranes and regulate membrane traffic in a PP1c-independent manner, suggesting a novel link between translation and traffic. MW of PPP1R15B is 80 kDa. Observed MW of PPP1R15B is from 100-110 kDa maybe due to phosphorylation (PMID: 28492545).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PPP1R15B antibody 14634-1-AP | Download protocol |
| IP protocol for PPP1R15B antibody 14634-1-AP | Download protocol |
| WB protocol for PPP1R15B antibody 14634-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The phospho-eIF2α antibody (Cat# 14634-1-AP) has 32 total citations published in journals including Nature, Science, Cell, Blood, Nat Commun, Neuron, Mol Cell, PNAS, Sci Adv, J Biol Chem, Hepatology, Hum Mol Genet, and others. Research fields covered include the integrated stress response, neurodegeneration, cancer (hepatocellular carcinoma, glioma), metabolic disorders, ER stress, translation regulation, wound healing, and mitochondrial dysfunction.
| Species | Application | Title |
|---|---|---|
Science Harnessing viral strategies to reverse cognitive dysfunction through the integrated stress response. | ||
Blood Eukaryotic initiation factor 2α phosphorylation mediates fetal hemoglobin induction through a post-transcriptional mechanism. | ||
Nat Commun Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis. | ||
Nat Commun Phosphorylation-coupled autoregulation of TANGO1 and Sec16A maintains functional ER exit sites. |
Reviews
What customers say
Both reviewers report positive experiences. One user (5/5) validated the antibody by Western blot, IHC, and immunofluorescence, detecting a major ~100 kDa band with upregulation in MG132-stressed cells, plus a double band at ~55 kDa. Specific signal was also confirmed in mouse CNS tissue (IHC) and human SH-SY5Y cells (ICC) at a 1:1000 dilution. Another user (4/5) used it successfully on hepatocytes by Western blot, noting it was colleague-recommended and that they would order again. Overall, users find it reliable across multiple applications and species.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tatyana (Verified Customer) (03-11-2023) | Detects a major band ~100 kDa and shows upregulation in stressed cells (MG132 treatment). WB: 4% milk in TBST and semi-dry transfer; detects a double band at ~55kDa. Also was tested in IHC in mouse CNS tissue and in ICC in human cells; the signal appears specific in both cases (not shown).
 |
Will (Verified Customer) (10-24-2019) | good antibody, recommended by a colleague, would order again
|
