PPP1R16B Polyclonal antibody

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  WB analysis using 25357-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25357-1-AP (PPP1R16B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

PPP1R16B Polyclonal Antibody for WB, ELISA

Cat No. 25357-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Synonyms

TIMAP, ANKRD4

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25357-1-AP targets PPP1R16B in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP1R16B fusion protein Ag19269
• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 16B
• Calculated Molecular Weight: 567 aa, 64 kDa
• Observed Molecular Weight: 60 kDa, 70 kDa
• GenBank Accession Number: BC131801
• Gene Symbol: PPP1R16B
• Gene ID (NCBI): 26051
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96T49
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP1R16B (Protein Phosphatase 1 Regulatory Subunit 16B), also known as TIMAP or ANKRD4, is a membrane-associated protein that acts as a regulator of protein phosphatase 1 (PP1). It contains five ankyrin repeats, a PP1-interacting domain, and a C-terminal CAAX box domain. The synthesis of PPP1R16B is inhibited by transforming growth factor-beta-1 (TGF-β-1), and it is predominantly expressed in endothelial cells. It has 2 isoforms with the molecular mass of 59 and 64 kDa.