PPP1R8 Polyclonal antibody

PPP1R8 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16115-1-AP

Synonyms

ARD 1, ARD1, NIPP 1, NIPP1, PPP1R8, PRO2047



Tested Applications

Positive WB detected inPC-3 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16115-1-AP targets PPP1R8 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP1R8 fusion protein Ag9041
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 8
Calculated molecular weight 209 aa, 23 kDa
Observed molecular weight 47 kDa, 28 kDa, 19 kDa
GenBank accession numberBC013360
Gene symbol PPP1R8
Gene ID (NCBI) 5511
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP1R8, also named as ARD1, NIPP1, is a 351 amino acid protein, which has a basic N- and C-terminal and an acidic central domain. PPP1R8 is ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. PPP1R8 has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates and may also be involved in pre-mRNA splicing. PPP1R8 exists many isoforms and molecular weight of modified isoforms is about 18 kDa, 28 kDa and 47 kDa.

Protocols

Product Specific Protocols
WB protocol for PPP1R8 antibody 16115-1-APDownload protocol
IHC protocol for PPP1R8 antibody 16115-1-APDownload protocol
IF protocol for PPP1R8 antibody 16115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols