PPP1R9B Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 85198-1-RR (same clone as 85198-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85198-1-RR (PPP1R9B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85198-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85198-1-RR (same clone as 85198-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85198-1-RR (PPP1R9B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85198-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 85198-1-RR (same clone as 85198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85198-1-RR (PPP1R9B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85198-1-PBS in a different storage buffer formulation.

PPP1R9B Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 85198-1-PBS

Clone No.242418G10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Neurabin 2, 242418G10, Neurabin II, Neurabin-2, Neurabin-II

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Product Information

85198-1-PBS targets PPP1R9B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 9B
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: NM_032595
• Gene Symbol: Spinophilin/Neurabin 2
• Gene ID (NCBI): 84687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96SB3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Neurabin 2, also named as Spinophilin, seems to act as a scaffold protein in multiple signaling pathways. It modulates excitatory synaptic transmission and dendritic spine morphology. PPP1R9B binds to actin filaments (F-actin) and shows cross-linking activity. It may play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. PPP1R9B plays a role in regulation of G-protein coupled receptor signaling, including D2Rs and alpha-adrenergic receptors. PPP1R9B probably regulates p70 S6 kinase activity by forming a complex with TIAM. The antibody is specific to PPP1R9B. The predicted molecular weight of spinophilin is 89 kDa, which differs significantly from the apparent MW seen in SDS/PAGE. Both the expressed full-length cDNA and the endogenous protein run at 120-130 kDa. This may be due to an extended conformation and/or low SDS binding capacity. (PMID: 28941770, PMID: 9275233).