- Featured Product
- KD/KO Validated
PPP2CA Polyclonal antibody
PPP2CA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, FC, CoIP, ELISA
Cat no : 13482-1-AP
Validation Data Gallery
|Positive WB detected in||Jurkat cells, human liver tissue, MCF-7 cells, HEK-293T cells|
|Positive IP detected in||Hela cells, Jurkat cells|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 5 publications below|
|WB||See 24 publications below|
|IHC||See 1 publications below|
|IF||See 2 publications below|
|IP||See 3 publications below|
|CoIP||See 1 publications below|
13482-1-AP targets PPP2CA in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PPP2CA fusion protein Ag4294|
|Full Name||protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform|
|Calculated molecular weight||309 aa, 36 kDa|
|Observed molecular weight||36 kDa|
|GenBank accession number||BC031696|
|Gene ID (NCBI)||5515|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.
|Product Specific Protocols|
|WB protocol for PPP2CA antibody 13482-1-AP||Download protocol|
|IHC protocol for PPP2CA antibody 13482-1-AP||Download protocol|
|IF protocol for PPP2CA antibody 13482-1-AP||Download protocol|
|IP protocol for PPP2CA antibody 13482-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.
Nuclear UHRF1 is a gate-keeper of cellular AMPK activity and function.
J Pineal Res
Melatonin increases susceptibility to atrial fibrillation in obesity via Akt signaling impairment in response to lipid overload
Sustained IKKβ phosphorylation and NF-κB activation by superoxide-induced peroxynitrite-mediated nitrotyrosine modification of B56γ3 and PP2A inactivation.
Cell Death Differ
The proteasome activator REGγ accelerates cardiac hypertrophy by declining PP2Acα-SOD2 pathway.
Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.