PPP2CA Polyclonal antibody

• Featured Product
• KD/KO Validated

PPP2CA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

34

Cat no : 13482-1-AP

Synonyms

Replication protein C, PP2CA, PP2Ac, PP2A-alpha, PP2A alpha

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Validation Data Gallery

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  WB analysis using 13482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13482-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13482-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13482-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 13482-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 13482-1-AP

  IP result of anti-PPP2CA (IP:13482-1-AP, 4ug; Detection:13482-1-AP 1:500) with Jurkat cells lysate 2400ug.

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  IHC staining of human liver using 13482-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 13482-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MCF-7 using 13482-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPP2CA antibody (13482-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of MCF-7 using 13482-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PPP2CA (13482-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HEK-293T cells, human liver tissue, Jurkat cells, MCF-7 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 32 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Product Information

13482-1-AP targets PPP2CA in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP2CA fusion protein Ag4294
• Full Name: protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
• Calculated Molecular Weight: 309 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC031696
• Gene Symbol: PPP2CA
• Gene ID (NCBI): 5515
• RRID: AB_2169485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.

Publications

Species	Application	Title
mouse	WB	Science; Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.; Authors - Hai Zheng
mouse	WB	Protein Cell; Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice; Authors - He-Zhou Huang
human	WB,IP	Cell Res; Nuclear UHRF1 is a gate-keeper of cellular AMPK activity and function.; Authors - Xiang Xu ; KD Validated
human	WB	Acta Pharm Sin B; WSB1 regulates c-Myc expression through β-catenin signaling and forms a feedforward circuit.; Authors - Xiaomeng Gao
mouse	WB	Cardiovasc Res; Role of type 2A phosphatase regulatory subunit B56α in regulating cardiac responses to β-adrenergic stimulation in vivo.; Authors - Sarah-Lena Puhl
human	WB	EMBO J; The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network; Authors - Cuirong Guan