|Positive WB detected in||Jurkat cells, human liver tissue, MCF-7 cells, HEK-293T cells|
|Positive IP detected in||Hela cells, Jurkat cells|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13482-1-AP targets PPP2CA in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PPP2CA fusion protein Ag4294|
|Full Name||protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform|
|Calculated molecular weight||309 aa, 36 kDa|
|Observed molecular weight||36 kDa|
|GenBank accession number||BC031696|
|Gene ID (NCBI)||5515|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.
|Product Specific Protocols|
|WB protocol for PPP2CA antibody 13482-1-AP||Download protocol|
|IHC protocol for PPP2CA antibody 13482-1-AP||Download protocol|
|IF protocol for PPP2CA antibody 13482-1-AP||Download protocol|
|IP protocol for PPP2CA antibody 13482-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Role of type 2A phosphatase regulatory subunit B56α in regulating cardiac responses to β-adrenergic stimulation in vivo.
ACS Chem Neurosci
Vitamin D Attenuates Alzheimer-like Pathology Induced by Okadaic Acid.
Cell Death Dis
Programmed cell death 10 promotes metastasis and epithelial-mesenchymal transition of hepatocellular carcinoma via PP2Ac-mediated YAP activation.
Acta Pharmacol Sin
Post-transcriptional modulation of protein phosphatase PPP2CA and tumor suppressor PTEN by endogenous siRNA cleaved from hairpin within PTEN mRNA 3'UTR in human liver cells.
J Cell Biochem
Reactivating PP2A by FTY720 as a novel therapy for AML with C-KIT tyrosine kinase domain mutation.
MFHAS1 suppresses TLR4 signaling pathway via induction of PP2A C subunit cytoplasm translocation and inhibition of c-Jun dephosphorylation at Thr239.