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PPP2CA Polyclonal antibody

PPP2CA Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 13482-1-AP


PP2A alpha, PP2Ac, PP2CA, PPP2CA, Replication protein C, RP C

Tested Applications

Positive WB detected inHEK-293T cells, human liver tissue, Jurkat cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13482-1-AP targets PPP2CA in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP2CA fusion protein Ag4294
Full Name protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
Calculated Molecular Weight 309 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC031696
Gene Symbol PPP2CA
Gene ID (NCBI) 5515
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.


Product Specific Protocols
WB protocol for PPP2CA antibody 13482-1-APDownload protocol
IHC protocol for PPP2CA antibody 13482-1-APDownload protocol
IF protocol for PPP2CA antibody 13482-1-APDownload protocol
IP protocol for PPP2CA antibody 13482-1-APDownload protocol
FC protocol for PPP2CA antibody 13482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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