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  • KD/KO Validated

PPP2CA Polyclonal antibody

PPP2CA Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 13482-1-AP

Synonyms

PP2A alpha, PP2Ac, PP2CA, PPP2CA, Replication protein C, RP C



Tested Applications

Positive WB detected inJurkat cells, human liver tissue, MCF-7 cells, HEK-293T cells
Positive IP detected inHela cells, Jurkat cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13482-1-AP targets PPP2CA in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP2CA fusion protein Ag4294
Full Name protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
Calculated molecular weight 309 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC031696
Gene symbol PPP2CA
Gene ID (NCBI) 5515
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.

Protocols

Product Specific Protocols
WB protocol for PPP2CA antibody 13482-1-APDownload protocol
IHC protocol for PPP2CA antibody 13482-1-APDownload protocol
IF protocol for PPP2CA antibody 13482-1-APDownload protocol
IP protocol for PPP2CA antibody 13482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cardiovasc Res

Role of type 2A phosphatase regulatory subunit B56α in regulating cardiac responses to β-adrenergic stimulation in vivo.

Authors - Sarah-Lena Puhl
mouse,humanWB

ACS Chem Neurosci

Vitamin D Attenuates Alzheimer-like Pathology Induced by Okadaic Acid.

Authors - Yiming Pan
WB

Acta Pharmacol Sin

Post-transcriptional modulation of protein phosphatase PPP2CA and tumor suppressor PTEN by endogenous siRNA cleaved from hairpin within PTEN mRNA 3'UTR in human liver cells.

Authors - Yu-En Gao
humanWB

J Cell Biochem

Reactivating PP2A by FTY720 as a novel therapy for AML with C-KIT tyrosine kinase domain mutation.

Authors - Yang Yan Y
humanWB

Mol Immunol

MFHAS1 suppresses TLR4 signaling pathway via induction of PP2A C subunit cytoplasm translocation and inhibition of c-Jun dephosphorylation at Thr239.

Authors - Qiqing Shi
mouseWB

Int J Mol Sci

PP2Ac Modulates AMPK-Mediated Induction of Autophagy in Mycobacterium bovis-Infected Macrophages.

Authors - Tariq Hussain