PPP2CB Polyclonal antibody

• Featured Product
• KD/KO Validated

PPP2CB Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, CoIP,ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12554-2-AP

Synonyms

PP2A beta, PP2CB, PPP2CB

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Validation Data Gallery

• 

  WB analysis of mouse lung using 12554-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12554-2-AP (PPP2CB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 12554-2-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 12554-2-AP (PPP2CB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 12554-2-AP

  IP result of anti-PPP2CB (IP:12554-2-AP, 4ug; Detection:12554-2-AP 1:300) with NIH/3T3 cells lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse lung tissue, U2OS cells
• Positive IP detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• CoIP: See 1 publications below

Product Information

12554-2-AP targets PPP2CB in WB, IP, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP2CB fusion protein Ag3233
• Full Name: protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform
• Calculated Molecular Weight: 309 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC012022
• Gene Symbol: PPP2CB
• Gene ID (NCBI): 5516
• RRID: AB_2169651
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,CoIP	Cell Mol Life Sci; GADD45α alleviates acetaminophen-induced hepatotoxicity by promoting AMPK activation.; Authors - Chunmin Li ; KD Validated
human	WB	Front Cell Dev Biol; BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells.; Authors - Baoyu Chen