Tested Applications
| Positive WB detected in | HepG2 cells, A431 cells, mouse kidney tissue, rat liver tissue, SMMC-7721 cells, mouse liver tissue, L02 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | mouse kidney tissue, human kidney tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15882-1-AP targets PPP2R1A in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8661 Product name: Recombinant human PPP2R1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 239-589 aa of BC001537 Sequence: DLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA Predict reactive species |
| Full Name | protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform |
| Calculated Molecular Weight | 589 aa, 65 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC001537 |
| Gene Symbol | PPP2R1A |
| Gene ID (NCBI) | 5518 |
| RRID | AB_2237574 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30153 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PPP2R1A belongs to the phosphatase 2A regulatory subunit A family. PPP2R1A encodes the α-isoform of the scaffolding subunit of the serine/threonine protein phosphatase 2A (PP2A) holoenzyme. This tumour suppressor complex is involved in growth and survival pathways and PPP2R1A mutations in endometrial high-grade serous carcinomas are a frequent and potentially targetable feature of this disease(PMID: 21381030). The PPP2R1A gene has 86% identity with the PPP2R1B gene(PMID:7681822).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PPP2R1A antibody 15882-1-AP | Download protocol |
| IHC protocol for PPP2R1A antibody 15882-1-AP | Download protocol |
| IP protocol for PPP2R1A antibody 15882-1-AP | Download protocol |
| WB protocol for PPP2R1A antibody 15882-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PPP2R1A Polyclonal antibody (Cat# 15882-1-AP) has 17 total citations. These appear in journals including Science, Nature Communications, Molecular Cell, Developmental Cell, Cell Reports, Cell Death Discovery, Acta Pharmaceutica Sinica B, Acta Neuropathologica Communications, British Journal of Pharmacology, Cancer Cell International, Frontiers in Cell and Developmental Biology, Bioorganic Chemistry, Journal of Virology, Microbiology Spectrum, Toxicology and Applied Pharmacology, Experimental Cell Research, and Journal of Cancer Research and Clinical Oncology. Research fields span transcription regulation, cancer, antiviral immunity, autophagy, and signaling pathways.
| Species | Application | Title |
|---|---|---|
Science Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase. | ||
Mol Cell SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape. | ||
Acta Pharm Sin B STK39 inhibits antiviral immune response by inhibiting DCAF1-mediated PP2A degradation | ||
Cell Death Discov Venlafaxine antagonizes the noradrenaline-promoted colon cancer progression by inhibiting the norepinephrine transporter
| ||
Dev Cell Fam72a functions as a cell-cycle-controlled gene during proliferation and antagonizes apoptosis through reprogramming PP2A substrates |
Reviews
What customers say
All three reviews are for Western Blot applications and are positive. One user reported excellent performance on A549 cell lysates at 1:3000 dilution. Another confirmed reliable detection of PPP2R1A in HEK293 and U2OS cell extracts at 1:2000. A third user noted strong results on mouse heart tissue at 1:1000. Overall, the antibody is consistently rated 4–5 stars, with users highlighting clear, specific signal across multiple cell lines and tissue types at dilutions ranging from 1:1000 to 1:3000.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shile (Verified Customer) (02-25-2026) | PPP2R1A Polyclonal antibody (Cat No. 15882-1-AP) was working very well in our WB experiments.
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Wissem (Verified Customer) (07-30-2024) | antibody works too detect PPP2R1A in HEK293 and U2OS cell extracts
|
Zee (Verified Customer) (01-28-2020) | It worked very well for western blot.
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