PPP2R1B Polyclonal antibody, PBS Only

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• PPP2R1B Polyclonal antibody, PBS Only (12621-1-PBS)

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  WB analysis using 12621-1-AP (same clone as 12621-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12621-1-AP (PPP2R1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12621-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12621-1-AP (same clone as 12621-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12621-1-AP (PPP2R1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12621-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 12621-1-AP (same clone as 12621-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12621-1-AP (PPP2R1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12621-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 12621-1-AP (same clone as 12621-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12621-1-AP (PPP2R1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12621-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 12621-1-AP (same clone as 12621-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12621-1-AP (PPP2R1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12621-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 12621-1-AP (same clone as 12621-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using PPP2R1B antibody (12621-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12621-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PPP2R1B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 12621-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

12621-1-PBS targets PPP2R1B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3307

  Product name: Recombinant human PPP2R1B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 320-667 aa of BC027596

  Sequence: ELGENLPIEDRETIIMNQILPYIKELVSDTNQHVKSALASVIMGLSTILGKENTIEHLLPLFLAQLKDECPDVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATNNLMKLVQKFGTEWAQNTIVPKVLVMANDPNYLHRMTTLFCINALSEACGQEITTKQMLPIVLKMAGDQVANVRFNVAKSLQKIGPILDTNALQGEVKPVLQKLGQDEDMDVKYFAQEAISVVAQRLRKLEFPVKDSGEPSVPRADKNHFPRPTVPGEDMGKGPVYQLRGDTRDTLAQLGIAELVHFSQSTD

• Full Name: protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform
• Calculated Molecular Weight: 667 aa, 74 kDa
• Observed Molecular Weight: 60-74 kDa
• GenBank Accession Number: BC027596
• Gene Symbol: PPP2R1B
• Gene ID (NCBI): 5519
• RRID: AB_2169941
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P30154
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.