PPP2R5E Polyclonal antibody

• Home
• Products
• Primary Antibodies
• PPP2R5E Polyclonal antibody (23885-1-AP)

PPP2R5E Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 23885-1-AP Copied!

Publications (1)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PP2A B subunit isoform B56-epsilon, PP2A B subunit isoform B'-epsilon, PP2A B subunit isoform PR61-epsilon, PP2A B subunit isoform R5-epsilon, Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform

• 

  WB analysis using 23885-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23885-1-AP (PPP2R5E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cervical cancer using 23885-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 23885-1-AP (PPP2R5E Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cervical cancer using 23885-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 23885-1-AP (PPP2R5E Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 23885-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23885-1-AP (PPP2R5E antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for PPP2R5E in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HT-1080 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23885-1-AP targets PPP2R5E in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20891

  Product name: Recombinant human PPP2R5E protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 118-467 aa of BC092477

  Sequence: MVSCNIFRTLPPSDSNEFDPEEDEPTLEASWPHLQLVYEFFIRFLESQEFQPSIAKKYIDQKFVLQLLELFDSEDPRERDYLKTVLHRIYGKFLGLRAFIRKQINNIFLRFVYETEHFNGVAELLEILGSIINGFALPLKAEHKQFLVKVLIPLHTVRSLSLFHAQLAYCIVQFLEKDPSLTEPVIRGLIKFWPKTCSQKEVMFLGELEEILDVIEPSQFVKIQEPLFKQIAKCVSSPHFQVAERALYYWNNEYIMSLIEENSNVILPIMFSSLYRISKEHWNPAIVALVYNVLKAFMEMNSTMFDELTATYKSDRQREKKKEKEREELWKKLEDLELKRGLRRDGIIPT

• Full Name: protein phosphatase 2, regulatory subunit B', epsilon isoform
• Calculated Molecular Weight: 467 aa, 55 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC092477
• Gene Symbol: PPP2R5E
• Gene ID (NCBI): 5529
• RRID: AB_2879350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q16537
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The PPP2R5E Polyclonal antibody (Cat# 23885-1-AP) has 1 total citation. It appears in Protein Cell (IF 18.2, 2024), where it was used by Western blot in a mouse study investigating how Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice. The associated research field is Alzheimer's disease and neurodegeneration.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Protein Cell; Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice; Authors - He-Zhou Huang