PPP3CB Polyclonal antibody

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• PPP3CB Polyclonal antibody (13340-1-AP)

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PPP3CB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13340-1-AP Copied!

Publications (17)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CALNA2, Calmodulin-dependent calcineurin A subunit beta isoform, CNA beta, CAM-PRP catalytic subunit, CAM PRP catalytic subunit

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  WB analysis using 13340-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13340-1-AP (PPP3CB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HEK-293 using 13340-1-AP

  WB result of PPP3CB antibody (13340-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP3CB transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human heart using 13340-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13340-1-AP (PPP3CB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 13340-1-AP

  IP result of anti-PPP3CB (IP:13340-1-AP, 4ug; Detection:13340-1-AP 1:2000) with mouse brain tissue lysate 1680 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of mouse brain using 13340-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13340-1-AP (PPP3CB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 13340-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13340-1-AP (PPP3CB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 13340-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13340-1-AP (PPP3CB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 13340-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13340-1-AP (PPP3CB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U-251 using 13340-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using PPP3CB antibody (13340-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, human heart tissue, HEK-293 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse kidney tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13340-1-AP targets PPP3CB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4163

  Product name: Recombinant human PPP3CB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-311 aa of BC028049

  Sequence: MAAPEPARAAPPPPPPPPPPPGADRVVKAVPFPPTHRLTSEEVFDLDGIPRVDVLKNHLVKEGRVDEEIALRIINEGAAILRREKTMIEVEAPITVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWVLKILYPSTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYEACMEAFDSLPLAALLNQQFLCVHGGLSPEIHTLDDIRRLDRFKEPPAFGPMCDLLWSDPSEDFGNEKSQEHFSHNTVRGCSYFYNYPAVCEFLQNNNLLSIIRAHEAQDAGYRMYRKSQTTGFPSL

• Full Name: protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform
• Calculated Molecular Weight: 525 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC028049
• Gene Symbol: PPP3CB
• Gene ID (NCBI): 5532
• RRID: AB_2284217
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P16298
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The antibody is a Rabbit Monoclonal Antibody targeting PPP3CB (Calcineurin B), catalog number 13340-1-AP. It has been cited in 17 publications across journals including Gastroenterology, Autophagy, Cell Death & Differentiation, PNAS, Oncogene, Advanced Science, and Proteomics. Research fields span hepatocellular carcinoma, pancreatic cancer, autophagy and lysosomal biogenesis, cardiac hypertrophy, sleep regulation, acute myeloid leukemia, osteoclast differentiation, Wnt signaling, and viral infection proteomics.

Automatically generated from the 17 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Gastroenterology; Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.; Authors - Haojie Jin
human	WB,IP	Autophagy; SMURF1 mediates damaged lysosomal homeostasis by ubiquitinating PPP3CB to promote the activation of TFEB; Authors - Qin Xia
human	IF	Autophagy; SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis; Authors - Qin Xia
pig	WB	Adv Sci (Weinh); Multi-Omics Insights Into the Mechanisms of Early Muscle Fiber Difference and Transformation Between Lean-Type and Chinese Indigenous Pigs.; Authors - Shuailong Zheng
human	IP	Cell Death Differ; The Smad4-MYO18A-PP1A complex regulates β-catenin phosphorylation and pemigatinib resistance by inhibiting PAK1 in cholangiocarcinoma.; Authors - Jialiang Liu
		Cell Death Differ; Schizophrenia and bipolar disorder risk gene AKAP11 sustains cognitive function by regulating TFEB-mediated autophagy.; Authors - Zhiqiang Deng