PPP3CB Polyclonal antibody

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  WB analysis using 13340-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13340-1-AP (PPP3CB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13340-1-AP

  WB result of PPP3CB antibody (13340-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP3CB transfected HEK-293 cells.

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  WB analysis of human heart using 13340-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13340-1-AP (PPP3CB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13340-1-AP

  IP result of anti-PPP3CB (IP:13340-1-AP, 4ug; Detection:13340-1-AP 1:2000) with mouse brain tissue lysate 1680 ug.

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  IHC staining of mouse brain using 13340-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13340-1-AP (PPP3CB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 13340-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13340-1-AP (PPP3CB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13340-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13340-1-AP (PPP3CB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13340-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13340-1-AP (PPP3CB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 13340-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using PPP3CB antibody (13340-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

PPP3CB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13340-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CALNA2, Calmodulin-dependent calcineurin A subunit beta isoform, CNA beta, CAM-PRP catalytic subunit, CAM PRP catalytic subunit

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Tested Applications

• Positive WB detected in: mouse brain tissue, human heart tissue, HEK-293 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse kidney tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

13340-1-AP targets PPP3CB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4163

  Product name: Recombinant human PPP3CB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-311 aa of BC028049

  Sequence: MAAPEPARAAPPPPPPPPPPPGADRVVKAVPFPPTHRLTSEEVFDLDGIPRVDVLKNHLVKEGRVDEEIALRIINEGAAILRREKTMIEVEAPITVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWVLKILYPSTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYEACMEAFDSLPLAALLNQQFLCVHGGLSPEIHTLDDIRRLDRFKEPPAFGPMCDLLWSDPSEDFGNEKSQEHFSHNTVRGCSYFYNYPAVCEFLQNNNLLSIIRAHEAQDAGYRMYRKSQTTGFPSL

• Full Name: protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform
• Calculated Molecular Weight: 525 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC028049
• Gene Symbol: PPP3CB
• Gene ID (NCBI): 5532
• RRID: AB_2284217
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P16298
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Gastroenterology; Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.; Authors - Haojie Jin
human	IF	Autophagy; SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis; Authors - Qin Xia
human	IP	Cell Death Differ; The Smad4-MYO18A-PP1A complex regulates β-catenin phosphorylation and pemigatinib resistance by inhibiting PAK1 in cholangiocarcinoma.; Authors - Jialiang Liu
human	WB	Oncogene; Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells.; Authors - Mengyi Lao ; KD Validated
		Am J Physiol Renal Physiol; TNF inhibits NKCC2 phosphorylation by a calcineurin-dependent pathway; Authors - Shoujin Hao
		Sci Rep; Calcineurin regulates cyclin D1 stability through dephosphorylation at T286.; Authors - Takahiro Goshima