PPP3R1 Polyclonal antibody

PPP3R1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 13210-1-AP

Synonyms

CNB1, CNB, CNA2, CALNB1, Calcineurin subunit B type 1

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Validation Data Gallery

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  WB analysis of mouse brain using 13210-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13210-1-AP (PPP3R1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 13210-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13210-1-AP (PPP3R1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 13210-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13210-1-AP (PPP3R1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13210-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13210-1-AP (PPP3R1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13210-1-AP

  IP result of anti-PPP3R1 (IP:13210-1-AP, 3ug; Detection:13210-1-AP 1:300) with HeLa cells lysate 5000ug.

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  IHC staining of human brain using 13210-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13210-1-AP (PPP3R1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13210-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13210-1-AP (PPP3R1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, human stomach tissue, K-562 cells, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 1 publications below

Product Information

13210-1-AP targets PPP3R1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP3R1 fusion protein Ag3992
• Full Name: protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform
• Calculated Molecular Weight: 170 aa, 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC027913
• Gene Symbol: PPP3R1
• Gene ID (NCBI): 5534
• RRID: AB_2252760
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein phosphatase 3 (PPP3, formerly PP2B, Calcineurin), a serine/ threonine protein phosphatase, is a heterodimer composed of one catalytic subunit (PPP3C, Calcineurin A) and one regulatory subunit (PPP3R, Calcineurin B). PPP3R has two isoforms, PPP3R1 and PPP3R2. PPP3Rl is ubiquitously expressed in different tissues and is coded by the PPP3R1 gene which was mapped recently to human chromosome 2. PPP3Rl has four functional calcium-binding sites.

Publications

Species	Application	Title
human	WB	Acta Biomater; Substrate stiffness regulates differentiation of induced pluripotent stem cells into heart valve endothelial cells.; Authors - Hong Cao
mouse	WB	Proc Natl Acad Sci U S A; Major contribution of the 3/6/7 class of TRPC channels to myocardial ischemia/reperfusion and cellular hypoxia/reoxygenation injuries.; Authors - Xiju He
human	WB	Biochem Biophys Res Commun; The calcineurin B subunit induces TNF-related apoptosis-inducing ligand (TRAIL) expression via CD11b-NF-κB pathway in RAW264.7 macrophages.; Authors - Su Zhenyi Z
chicken	WB	Biol Trace Elem Res; Transcriptional Study Revealed That Boron Supplementation May Alter the Immune-Related Genes Through MAPK Signaling in Ostrich Chick Thymus.; Authors - Ke Xiao
mouse	WB	Autophagy; Narirutin activates TFEB (transcription factor EB) to protect against acetaminophen-induced liver injury by targeting PPP3/calcineurin; Authors - Zhiyuan Fang
human	IF	Autophagy; SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis; Authors - Qin Xia