Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14005-1-AP targets PPP3R2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5097 Product name: Recombinant human PPP3R2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-173 aa of BC030595 Sequence: MSTMGNEASYPAEMCSHFDNDEIKRLGRRFKKLDLDKSGSLSVEEFMSLPELRHNPLVRRVIDVFDTDGDGEVDFKEFILGTSQFSVKGDEEQKLRFAFSIYDMDKDGYISNGELFQVLKMMVGNNLTDWQLQQLVDKTIIILDKDGDGKISFEEFSAVVRDLEIHKKLVLIV Predict reactive species |
| Full Name | protein phosphatase 3 (formerly 2B), regulatory subunit B, beta isoform |
| Calculated Molecular Weight | 20 kDa |
| Observed Molecular Weight | 20 kDa |
| GenBank Accession Number | BC030595 |
| Gene Symbol | PPP3R2 |
| Gene ID (NCBI) | 5535 |
| RRID | AB_2168590 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96LZ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PPP3R2 (protein phosphatase 3 regulatory subunit B, beta) encodes the regulatory subunit B type 2 of calcineurin, a calcium/calmodulin-dependent serine/threonine protein phosphatase. As a member of the calcineurin B subunit family, PPP3R2 contains EF-hand calcium-binding domains and confers calcium sensitivity to the phosphatase complex. Unlike the ubiquitously expressed PPP3R1, PPP3R2 is expressed almost exclusively in the testis, where it assembles with the catalytic subunit PPP3CC to form a testis-enriched "sperm calcineurin". In mature spermatozoa, this complex is localized to the mitochondrial sheath of the midpiece and the flagellum, and it is essential for proper sperm motility, structural integrity of the midpiece, and male fertility.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PPP3R2 antibody 14005-1-AP | Download protocol |
| IP protocol for PPP3R2 antibody 14005-1-AP | Download protocol |
| WB protocol for PPP3R2 antibody 14005-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PPP3R2 Polyclonal antibody (Cat# 14005-1-AP) has 4 citations. These appear in Cell Death & Disease, PNAS, European Journal of Pharmacology, and FASEB Journal. The cited research spans osteoarthritis and cartilage homeostasis, mitochondrial calcineurin regulation of sperm motility, calcineurin/NFAT signaling in cardiac hypertrophy, and calcineurin's role in sperm fertilization. All citations used mouse samples with applications including WB, IF, and IHC.
| Species | Application | Title |
|---|---|---|
Cell Death Dis miR-146a facilitates osteoarthritis by regulating cartilage homeostasis via targeting Camk2d and Ppp3r2. | ||
Proc Natl Acad Sci U S A SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. | ||
Eur J Pharmacol Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway. | ||
FASEB J Roles of glycogen synthase kinase 3 alpha and calcineurin in regulating the ability of sperm to fertilize eggs. |
