Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, K-562 cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11715-1-AP targets PPP5C in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2288 Product name: Recombinant human PPP5C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 150-500 aa of BC001970 Sequence: RAIAGDEHKRSVVDSLDIESMTIEDEYSGPKLEDGKVTISFMKELMQWYKDQKKLHRKCAYQILVQVKEVLSKLSTLVETTLKETEKITVCGDTHGQFYDLLNIFELNGLPSETNPYIFNGDFVDRGSFSVEVILTLFGFKLLYPDHFHLLRGNHETDNMNQIYGFEGEVKAKYTAQMYELFSEVFEWLPLAQCINGKVLIMHGGLFSEDGVTLDDIRKIERNRQPPDSGPMCDLLWSDPQPQNGRSISKRGVSCQFGPDVTKAFLEENNLDYIIRSHEVKAEGYEVAHGGRCVTVFSAPNYCDQMGNKASYIHLQGSDLRPQFHQFTAVPHPNVKPMAYANTLLQLGMM Predict reactive species |
| Full Name | protein phosphatase 5, catalytic subunit |
| Calculated Molecular Weight | 499 aa, 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC001970 |
| Gene Symbol | PPP5C |
| Gene ID (NCBI) | 5536 |
| RRID | AB_2237478 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P53041 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PPP5C antibody 11715-1-AP | Download protocol |
| WB protocol for PPP5C antibody 11715-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PPP5C antibody (Cat# 11715-1-AP) has 8 total citations published in journals including Immunity, J Adv Res, Int J Oncol, iScience, Onco Targets Ther, Oncol Lett, and Cancer Biother Radiopharm. Research fields span inflammation and immune regulation, cancer biology (bladder, prostate, pancreatic, and lung cancers), cell proliferation and apoptosis, and signaling pathway modulation (JNK/ERK, DDX5-ATF1). Applications include WB, IP, and IHC in mouse and human samples.
| Species | Application | Title |
|---|---|---|
Immunity Intestinal dysbiosis exacerbates skin inflammation via microbial metabolite-driven Th2 cell differentiation. | ||
J Adv Res Transducin-like enhancer of split 3 protects against lipopolysaccharide-induced inflammation through DDX5-ATF1-PPP2R5A signaling | ||
Int J Oncol Disruption of serine/threonine protein phosphatase 5 inhibits tumorigenesis of urinary bladder cancer cells. | ||
iScience TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment | ||
Onco Targets Ther PPP5C promotes cell proliferation and survival in human prostate cancer by regulating of the JNK and ERK1/2 phosphorylation.
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