PPP5C Polyclonal antibody

• Featured Product
• KD/KO Validated

PPP5C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11715-1-AP

Synonyms

PP T, PP5, PPP5, PPP5C, PPT, Protein phosphatase T

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Validation Data Gallery

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  WB analysis using 11715-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11715-1-AP (PPP5C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 11715-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11715-1-AP (PPP5C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11715-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11715-1-AP (PPP5C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, K-562 cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

11715-1-AP targets PPP5C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP5C fusion protein Ag2288
• Full Name: protein phosphatase 5, catalytic subunit
• Calculated Molecular Weight: 499 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC001970
• Gene Symbol: PPP5C
• Gene ID (NCBI): 5536
• RRID: AB_2237478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Int J Oncol; Disruption of serine/threonine protein phosphatase 5 inhibits tumorigenesis of urinary bladder cancer cells.; Authors - Ming Chen
human	WB	Oncol Lett; MicroRNA-365b-3p represses the proliferation and promotes the apoptosis of non-small cell lung cancer cells by targeting PPP5C.; Authors - Xiaomiao Zhang
human	WB,IHC	Onco Targets Ther; PPP5C promotes cell proliferation and survival in human prostate cancer by regulating of the JNK and ERK1/2 phosphorylation.; Authors - Jian-Min Lv ; KD Validated
		Cancer Biother Radiopharm; Overexpression of TSPAN8 Promotes Tumor Cell Viability and Proliferation in Nonsmall Cell Lung Cancer.; Authors - Zheng Dong
human	WB	Oncol Lett; PMID: 29805615; Authors - Jinhui Zhu ; KD Validated
human	WB	iScience; TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment; Authors - Magdalena Wolczyk