PPRC1 Polyclonal antibody

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  WB analysis of NIH/3T3 using 25429-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25429-1-AP (PPRC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25429-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25429-1-AP (PPRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PPRC1 Polyclonal Antibody for WB, ELISA

Cat No. 25429-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

KIAA0595, PGC 1 related coactivator, PPRC1, PRC, RP11 302K17.6

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

25429-1-AP targets PPRC1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPRC1 fusion protein Ag11940
• Full Name: peroxisome proliferator-activated receptor gamma, coactivator-related 1
• Calculated Molecular Weight: 1542 aa, 165 kDa
• Observed Molecular Weight: 177 kDa
• GenBank Accession Number: BC063806
• Gene Symbol: PPRC1
• Gene ID (NCBI): 23082
• RRID: AB_2880074
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5VV67
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peroxisome proliferator-activated receptor gamma, co-activator-related 1 (Pprc1) is the third member of the Pgc1 family, which contributes to mitochondrial biogenesis and orchestrates response to metabolic stress by promoting the expression of multiple genes specifying inflammation, proliferation, and metabolic reprogramming.

Publications

Species	Application	Title
human	WB	Cancer Res; PGC-1α degradation suppresses mitochondrial biogenesis to confer radiation resistance in glioma; Authors - Mengjie Zhao
human	WB	Sci Rep; CRIP1 inhibits cutaneous melanoma progression through TFAM-mediated mitochondrial biogenesis; Authors - Jianqiang Wu