Tested Applications
| Positive WB detected in | HEK-293 cells, A549 cells, HeLa cells, SH-SY5Y cells, mouse testis tissue, rat testis tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29653-1-AP targets PPT1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31240 Product name: Recombinant human PPT1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 150-306 aa of BC008426 Sequence: PRCPGESSHICDFIRKTLNAGAYSKVVQERLVQAEYWHDPIKEDVYRNHSIFLADINQERGINESYKKNLMALKKFVMVKFLNDSIVDPVDSEWFGFYRSGQAKETIPLQETSLYTQDRLGLKEMDNAGQLVFLATEGDHLQLSEEWFYAHIIPFLG Predict reactive species |
| Full Name | palmitoyl-protein thioesterase 1 |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 32-34 kDa |
| GenBank Accession Number | BC008426 |
| Gene Symbol | PPT1 |
| Gene ID (NCBI) | 5538 |
| RRID | AB_2923599 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P50897 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The defective gene behind the INCL(infantile neuronal ceroid lipofuscinoses) disease, CLN1, encodes for palmitoyl protein thioesterase 1 (PPT1). It consists of 306 amino acids, including a signal sequence of 26 amino acids and three N-linked glycosylation sites. This protein exists with a molecular mass of 32 kDa, 34 kDa,36 kDa, and 38 kDa. The enzyme is transported into lysosomes of non-neuronal cells by the mannose 6-phosphate receptor (M6PR) mediated pathway and a significant amount of native PPT1 resides in a complex rather than in a monomeric form(PMID:17565660).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PPT1 antibody 29653-1-AP | Download protocol |
| IHC protocol for PPT1 antibody 29653-1-AP | Download protocol |
| WB protocol for PPT1 antibody 29653-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PPT1 antibody (Cat# 29653-1-AP) has a total of 7 citations. Citations appear in Advanced Science (3), Cell Reports Medicine, Science Advances, Cell Death & Disease, and EMBO Reports. Research fields span lipid metabolism and protein palmitoylation/depalmitoylation, innate immunity, adipogenesis, macrophage autophagy, inflammation and wound healing, neuronal excitability and cognitive function, osteosarcoma, and TRPV1-mediated nociception. Applications include WB, IHC, and IF across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Palmitic Acid Promotes Antiviral Innate Immunity via ZDHHC20-Mediated CMPK2 Palmitoylation. | ||
Adv Sci (Weinh) PIK3CA Mutations Downregulate PPT1 to Promote Adipogenesis by Suppressing P300 Depalmitoylation and Phase Separation. | ||
Adv Sci (Weinh) Gut Metabolite Indole-3-Propionic Acid Regulates Macrophage Autophagy Through PPT1 Inhibiting Aging-Related Myocardial Fibrosis. | ||
Cell Rep Med Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing
| ||
Sci Adv PPT1 selectively depalmitoylates GAP43 to regulate neuronal excitability and cognitive function. | ||
Cell Death Dis RHBDL2 drives lipid metabolic reprogramming in osteosarcoma via USP3-mediated deubiquitination of PPT1.
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