PPT1 Polyclonal antibody

PPT1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10887-1-AP

Synonyms

CLN1, INCL, Palmitoyl protein hydrolase 1, PPT, PPT 1, PPT1

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Validation Data Gallery

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  IHC staining of human kidney using 10887-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10887-1-AP (PPT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10887-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10887-1-AP (PPT1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

10887-1-AP targets PPT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPT1 fusion protein Ag1131
• Full Name: palmitoyl-protein thioesterase 1
• Calculated Molecular Weight: 34 kDa
• GenBank Accession Number: BC008426
• Gene Symbol: PPT1
• Gene ID (NCBI): 5538
• RRID: AB_2168906
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The defective gene behind the INCL(infantile neuronal ceroid lipofuscinoses) disease, CLN1, encodes for palmitoyl protein thioesterase 1 (PPT1). It consists of 306 amino acids, including a signal sequence of 26 amino acids and three N-linked glycosylation sites. This protein exsits with the molecular mass of 32 kDa, 34 kDa,36 kDa and 38 kDa. The enzyme is transported into lysosomes of non-neuronal cells by the mannose 6-phosphate receptor (M6PR) mediated pathway and a significant amount of native PPT1 resides in a complex rather than in a monomeric form(PMID:17565660).

Publications

Species	Application	Title
human,mouse	WB,IHC	iScience; PPT1 regulation of HSP90α depalmitoylation participates in the pathogenesis of hyperandrogenism; Authors - Tongmin Xue