CoraLite® Plus 488-conjugated PQBP1 Recombinant antibody

• 

  IF Staining of HeLa using CL488-84491-6

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PQBP1 antibody (CL488-84491-6, Clone: 241901B11 ) at dilution of 1:200, CL594-Phalloidin (red).

PQBP1 Uni-rAb^TM Recombinant Antibody for IF/ICC

Cat No. CL488-84491-6

Clone No.241901B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

241901B11, Npw38, Polyglutamine tract-binding protein 1, Polyglutamine-binding protein 1, PQBP-1

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84491-6 targets PQBP1 in applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PQBP1 fusion protein Ag9329
• Full Name: polyglutamine binding protein 1
• Calculated Molecular Weight: 265 aa, 30 kDa
• Observed Molecular Weight: 30-32 kDa
• GenBank Accession Number: BC012358
• Gene Symbol: PQBP1
• Gene ID (NCBI): 10084
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60828
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PQBP1 (Polyglutamine-binding protein 1) is also named as NPW38 and JM26. PQBP1 senses extrinsic tau 3R/4R proteins by direct interaction and triggers an innate immune response by activating a cyclic GMP-AMP synthase (cGAS)-Stimulator of interferon genes (STING) pathway (PMID: 34782623).  PQBP1 is abundantly expressed in the central nervous system during development. PQBP1 is a component of the precatalytic spliceosome (B complex) that is involved in splicing. PQBP1 was predominantly localized in the cell nucleus. PQBP1 is overexpressed in various cancer types including breast cancer, colon cancer, and glioblastoma (PMID: 38342602).