PRAM1 Polyclonal antibody

PRAM1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13651-1-AP

Synonyms

PML RAR, PRAM 1, PRAM1

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Validation Data Gallery

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  WB analysis of U-937 using 13651-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13651-1-AP (PRAM1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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Tested Applications

• Positive WB detected in: U-937 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13651-1-AP targets PRAM1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRAM1 fusion protein Ag4162
• Full Name: PML-RARA regulated adaptor molecule 1
• Calculated Molecular Weight: 718 aa, 79 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC028012
• Gene Symbol: PRAM1
• Gene ID (NCBI): 84106
• RRID: AB_2877964
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRAM1, also named as PML-RARA-regulated adapter molecule 1, is a 718 amino acid protein, which contains 1 SH3 domain. PRAM1 is expressed in peripheral blood leukocytes and bone marrow. It is not expressed in non hematopoietic tissues except in lung. PRAM1 may be involved in myeloid differentiation and integrin signaling in neutrophils.