Tested Applications
| Positive WB detected in | U-937 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13651-1-AP targets PRAM1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4162 Product name: Recombinant human PRAM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-299 aa of BC028012 Sequence: MAHHLPAAMENHQDFRSIKAKFQASQPEPSDLPKKPPKPEFGKLKKFSQPELSEHPKKAPLPEFGAVSLKPPPPEVTDLPKKPPPPEVTDLPKKPPPPEVTDLPKKPPPPEVTDLPKKPSKLELSDLSKKFPQLGATPFPRKPLQPEVGEAPLKASLPEPGAPARKPLQPDELSHPARPPSEPKSGAFPRKLWQPEAGEATPRSPQPELSTFPKKPAQPEFNVYPKKPPQPQVGGLPKKSVPQPEFSEAAQTPLWKPQSSEPKRDSSAFPKKASQPPLSDFPKKPPQPELGDLTRTSSE Predict reactive species |
| Full Name | PML-RARA regulated adaptor molecule 1 |
| Calculated Molecular Weight | 718 aa, 79 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC028012 |
| Gene Symbol | PRAM1 |
| Gene ID (NCBI) | 84106 |
| RRID | AB_2877964 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96QH2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PRAM1, also named as PML-RARA-regulated adapter molecule 1, is a 718 amino acid protein, which contains 1 SH3 domain. PRAM1 is expressed in peripheral blood leukocytes and bone marrow. It is not expressed in non hematopoietic tissues except in lung. PRAM1 may be involved in myeloid differentiation and integrin signaling in neutrophils.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PRAM1 antibody 13651-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PRAM1 Polyclonal Antibody (Cat# 13651-1-AP) has 1 citation to date. It was cited in Discover Oncology (2026) in a study on lactylation-related gene signatures for prognosis prediction and immune infiltration assessment in lung adenocarcinoma using bulk and single-cell RNA sequencing. The antibody was applied in Western Blot (WB) with human samples. The associated research field is oncology, specifically lung adenocarcinoma and immune microenvironment analysis.
| Species | Application | Title |
|---|---|---|
Discov Oncol Lactylation-related gene signature for prognosis prediction and immune infiltration assessment in lung adenocarcinoma via bulk and single-cell RNA sequencing. |

