|Positive WB detected in||MCF-7 cells, HEK-293 cells, HeLa cells, K-562 cells, Raji cells, SH-SY5Y cells, C6 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
15617-1-AP targets PRC1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PRC1 fusion protein Ag8028|
|Full Name||protein regulator of cytokinesis 1|
|Calculated molecular weight||72 kDa|
|Observed molecular weight||66 kDa|
|GenBank accession number||BC003138|
|Gene ID (NCBI)||9055|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.
|Product Specific Protocols|
|WB protocol for PRC1 antibody 15617-1-AP||Download protocol|
|IHC protocol for PRC1 antibody 15617-1-AP||Download protocol|
|IF protocol for PRC1 antibody 15617-1-AP||Download protocol|
|IP protocol for PRC1 antibody 15617-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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