PRC1 Polyclonal antibody

PRC1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15617-1-AP

Synonyms

ASE1, PRC1



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15617-1-AP targets PRC1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRC1 fusion protein Ag8028
Full Name protein regulator of cytokinesis 1
Calculated molecular weight 72 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC003138
Gene symbol PRC1
Gene ID (NCBI) 9055
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.

Protocols

Product Specific Protocols
WB protocol for PRC1 antibody 15617-1-APDownload protocol
IHC protocol for PRC1 antibody 15617-1-APDownload protocol
IF protocol for PRC1 antibody 15617-1-APDownload protocol
IP protocol for PRC1 antibody 15617-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Proc Natl Acad Sci U S A

Oocyte stage-specific effects of MTOR determine granulosa cell fate and oocyte quality in mice.

Authors - Jing Guo
humanWB

Oncotarget

Piperlongumine promotes death of retinoblastoma cancer cells.

Authors - Nathalie Allaman-Pillet
mouseWB

Genes Cells

Cdk1 negatively regulates the spindle localization of Prc1 in mouse oocytes.

Authors - Sui Nishiyama
mouseIF

Mol Biol Cell

Chromokinesin Kif4 promotes proper anaphase in mouse oocyte meiosis.

Authors - Carissa M Heath
mouseWB,IF

FEBS J

PRC1 is a critical regulator for anaphase spindle midzone assembly and cytokinesis in mouse oocyte meiosis.

Authors - Xiao-Han Li
  • KD Validated
humanWB

Front Oncol

Identifying and Validating Potential Biomarkers of Early Stage Lung Adenocarcinoma Diagnosis and Prognosis.

Authors - Yingji Chen