PRDM5 Polyclonal antibody

PRDM5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14126-1-AP

Synonyms

PR domain-containing protein 5, PR domain containing protein 5, PR domain containing 5, PFM2, EC:2.1.1.-

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Validation Data Gallery

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  WB analysis of mouse ovary using 14126-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14126-1-AP (PRDM5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 14126-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14126-1-AP (PRDM5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 14126-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14126-1-AP (PRDM5 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse ovary tissue, mouse colon tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14126-1-AP targets PRDM5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRDM5 fusion protein Ag5277
• Full Name: PR domain containing 5
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC066942
• Gene Symbol: PRDM5
• Gene ID (NCBI): 11107
• RRID: AB_2878019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.