PRDX6 Recombinant monoclonal antibody

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  WB analysis using 86368-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86368-1-RR (PRDX6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86368-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86368-1-RR against Human PRDX6 were performed. The affinity constant is 0.234 nM.

PRDX6 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86368-1-RR

Clone No.251053E8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

1-Cys peroxiredoxin, 1-Cys PRX, Acidic calcium-independent phospholipase A2, AOP2, EC:1.11.1.27

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells, SH-SY5Y cells, A549 cells, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86368-1-RR targets PRDX6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4512

  Product name: Recombinant human PRDX6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-224 aa of BC035857

  Sequence: MPGGLLLGDVAPNFEANTTVGRIRFHDFLGDSWGILFSHPRDFTPVCTTELGRAAKLAPEFAKRNVKLIALSIDSVEDHLAWSKDINAYNCEEPTEKLPFPIIDDRNRELAILLGMLDPAEKDEKGMPVTARVVFVFGPDKKLKLSILYPATTGRNFDEILRVVISLQLTAEKRVATPVDWKDGDSVMVLPTIPEEEAKKLFPKGVFTKELPSGKKYLRYTPQP

• Full Name: peroxiredoxin 6
• Calculated Molecular Weight: 224 aa, 25 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC035857
• Gene Symbol: PRDX6
• Gene ID (NCBI): 9588
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30041
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).