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PREPL Polyclonal antibody, PBS Only (12478-1-PBS)

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WB analysis of human brain using 12478-1-AP (same clone as 12478-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 12478-1-AP (PREPL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12478-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 12478-1-AP (same clone as 12478-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12478-1-AP (PREPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12478-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12478-1-AP (same clone as 12478-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12478-1-AP (PREPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12478-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12478-1-AP (same clone as 12478-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12478-1-AP (PREPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12478-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 12478-1-AP (same clone as 12478-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12478-1-AP (PREPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12478-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 12478-1-AP (same clone as 12478-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12478-1-AP (PREPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12478-1-PBS in a different storage buffer formulation.

PREPL Polyclonal antibody, PBS Only

Name: PREPL Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 12478-1-PBS

PREPL Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 12478-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

EC:3.4.21.-, KIAA0436, PPCEL, prolyl endopeptidase like, Prolyl endopeptidase-like

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

12478-1-PBS targets PREPL in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3196 Product name: Recombinant human PREPL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-244 aa of BC013193 Sequence: MDLKMNFRPERRVLVDDGWILAYCHVRYGGELGLQWHADGRLTKKLNGLADLEACIKTLHGQGFSQPSLTTLTAFSAGGVLAGALCNSNPELVRAVTLEAPFLDVLNTMMDTTLPLTLEELEEWGNPSSDEKHKNYIKRYCPYQNIKPQHYPSIHITAYENDERVPLKGIVSYTEKLKEAIAEHAKDTGEGYQTPNIILDIQPGGNHVIEDSHKKITAQIKFLYEELGLDSTSVFEDLKKYLKF Predict reactive species
Full Name	prolyl endopeptidase-like
Calculated Molecular Weight	72 kDa
Observed Molecular Weight	72 kDa
GenBank Accession Number	BC013193
Gene Symbol	PREPL
Gene ID (NCBI)	9581
RRID	AB_2300040
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q4J6C6
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PREPL (Prolyl endopeptidase-like) is also named as KIAA0436 and belongs to the prolyl oligopeptidase subfamily of serine peptidases. IPREPL is a novel oligopeptidase with unique structural and functional characteristics (PMID:16385448). It has 4 isoforms produced by alternative splicing and can exsit as a homodimer (PMID:16143824). Defects in PREPL are a cause of hypotonia-cystinuria syndrome (HCS).