Tested Applications
| Positive WB detected in | MCF-7 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33193-1-AP targets PREX1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36649 Product name: Recombinant human PREX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 700-900 aa of NM_020820.3 Sequence: LLVATKAKEIIKIPDQPDTLCFQIRGAAPPYVYAVGRGSEAMAAGLCAGQCILKVNGSNVMNDGAPEVLEHFQAFRSRREEALGLYQWIYHTHEDAQEARASQEASTEDPSGEQAQEEDQADSAFPLLSLGPRLSLCEDSPMVTLTVDNVHLEHGVVYEYVSTAGVRCHVLEKIVEPRGCFGLTAKILEAFAANDSVFVEN Predict reactive species |
| Full Name | phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1 |
| Calculated Molecular Weight | 186 kDa |
| Observed Molecular Weight | 186 kDa |
| GenBank Accession Number | NM_020820.3 |
| Gene Symbol | PREX1 |
| Gene ID (NCBI) | 57580 |
| RRID | AB_3742888 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8TCU6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (PREX1) is a guanine nucleotide exchange factor (GEF) that specifically activates Rac GTPases, particularly Rac1 (PMID: 16243035). PREX1 is activated downstream of G protein-coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs), such as ErbB receptors, and is involved in PI3K/AKT and MAPK signaling pathways (PMID: 28710285; 27358402). It contributes to glucose uptake in adipocytes and promotes tumor cell proliferation, migration, and metastasis in various cancers, including breast, prostate, and melanoma (PMID: 22109529).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PREX1 antibody 33193-1-AP | Download protocol |
| WB protocol for PREX1 antibody 33193-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PREX1 Polyclonal antibody (Cat# 33193-1-AP) has 1 total citation. The cited publication appeared in Cell Signal and investigated PREX1's role in regulating renal fibrosis through the RPTOR-mTORC1 signaling pathway. The study utilized this antibody for WB, IHC, and CoIP applications in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Signal PREX1 regulates renal fibrosis through the RPTOR-mTORC1 signaling pathway.
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