PRIM2 Polyclonal antibody

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  WB analysis using 11788-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11788-1-AP (PRIM2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of A375 using 11788-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using 11788-1-AP (PRIM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

PRIM2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 11788-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

PRIM2A, p58

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Tested Applications

• Positive WB detected in: A375 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

11788-1-AP targets PRIM2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRIM2 fusion protein Ag2370
• Full Name: primase, DNA, polypeptide 2 (58kDa)
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC017833
• Gene Symbol: PRIM2
• Gene ID (NCBI): 5558
• RRID: AB_2169380
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49643
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Med Oncol; PRIM2 promotes proliferation and metastasis of pancreatic ductal adenocarcinoma through interactions with FAM111B; Authors - Jingyang Yin ; KD Validated